留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

遮阴对浙北地区七叶一枝花生理生化指标及皂苷含量的影响

陈文 刘守赞 耿东杰 顾依雯 李者 潘洁渝 白岩

陈文, 刘守赞, 耿东杰, 顾依雯, 李者, 潘洁渝, 白岩. 遮阴对浙北地区七叶一枝花生理生化指标及皂苷含量的影响[J]. 中国生态农业学报 (中英文), 2022, 30(1): 72−81 doi: 10.12357/cjea.20210323
引用本文: 陈文, 刘守赞, 耿东杰, 顾依雯, 李者, 潘洁渝, 白岩. 遮阴对浙北地区七叶一枝花生理生化指标及皂苷含量的影响[J]. 中国生态农业学报 (中英文), 2022, 30(1): 72−81 doi: 10.12357/cjea.20210323
CHEN W, LIU S Z, GENG D J, GU Y W, LI Z, PAN J Y, BAI Y. Effect of shading on saponin content and biochemical indexes of Paris polyphylla Smith var. chinensis (Franch.) Hara in northern Zhejiang[J]. Chinese Journal of Eco-Agriculture, 2022, 30(1): 72−81 doi: 10.12357/cjea.20210323
Citation: CHEN W, LIU S Z, GENG D J, GU Y W, LI Z, PAN J Y, BAI Y. Effect of shading on saponin content and biochemical indexes of Paris polyphylla Smith var. chinensis (Franch.) Hara in northern Zhejiang[J]. Chinese Journal of Eco-Agriculture, 2022, 30(1): 72−81 doi: 10.12357/cjea.20210323

遮阴对浙北地区七叶一枝花生理生化指标及皂苷含量的影响

doi: 10.12357/cjea.20210323
基金项目: 浙江省重点研发项目(2021C02043)和浙江省林业科研成果推广项目(2019B04)资助
详细信息
    作者简介:

    陈文, 研究方向为药用植物次生代谢调控, E-mail: chenwen@stu.zafu.edu.cn

    刘守赞, 研究方向为珍稀中药资源收集、整理与评价, E-mail: szliu@zafu.edu.cn

    通讯作者:

    白岩, 研究方向为药用植物次生代谢调控和中药资源整理与评价。E-mail: hzbaiyan@zafu.edu.cn

  • 中图分类号: R932

Effect of shading on saponin content and biochemical indexes of Paris polyphylla Smith var. chinensis (Franch.) Hara in northern Zhejiang

Funds: This study was supported by the Key Research and Development Program of Zhejiang Province (2021C02043) and the Forestry Scientific Research Extension Foundation of Zhejiang Province (2019B04)
More Information
  • 摘要: 七叶一枝花[Paris polyphylla Smith var. chinensis (Franch.) Hara]为药材重楼的药源植物, 具有较好的抗肿瘤、抗炎等作用; 野生七叶一枝花生于海拔1800~3200 m林下, 目前已资源匮乏, 人工栽培尚不成规模, 同时, 七叶一枝花生长受光强影响显著, 强度不适时植株易萎蔫死亡, 因此, 研究遮阴处理对人工培育优质七叶一枝花具有重要意义。本文以6年生的七叶一枝花为试验材料, 设置不同遮阴条件(70%、80%、90%), 对重楼皂苷含量、光合色素含量、抗氧化酶活性、MDA含量、可溶性物质含量等指标进行测定, 探究遮阴程度对七叶一枝花中皂苷成分的影响规律。结果显示, 重楼皂苷含量与SOD活性、POD活性、MDA含量存在显著负相关性(P<0.05), 与叶绿素a/b、CAT活性、可溶性蛋白质含量存在显著正相关(P<0.05); 在遮阴80%的处理下, 重楼皂苷含量最高(重楼皂苷Ⅰ为1.45 mg∙g−1, 重楼皂苷Ⅵ为2.52 mg∙g−1, 重楼皂苷Ⅶ为3.22 mg∙g−1, 总重楼皂苷为7.19 mg∙g−1); 同时该遮阴下各项光合色素指标和可溶性蛋白质含量(11.28 mg∙g−1)均为最高, POD (2.89 U∙g−1∙min−1)和SOD (105.01 U∙g−1∙min−1)活性最低, MDA (11.90 μmol∙g−1)和脯氨酸(FP, 8.88 mg∙g−1)含量最低。综上所述, 不同的遮阴程度对七叶一枝花中重楼皂苷含量和初生代谢影响较大; 其中80%遮阴处理有利于其重楼皂苷合成和积累, 有利于光合色素和可溶性蛋白质含量的提高; 较高或较低遮阴对七叶一枝花生长和重楼皂苷的含量均产生不利影响。本研究结果可为优化七叶一枝花人工栽培措施及获得优质药材提供理论参考。
  • 图  1  重楼皂苷标准品(A)和样品(B)的HPLC色谱图(1: 重楼皂苷Ⅶ; 2: 重楼皂苷Ⅵ; 3: 重楼皂苷Ⅱ; 4: 重楼皂苷Ⅰ)

    Figure  1.  HPLC chromatograms of polyphyllin standards and samples (1: polyphyllin Ⅶ; 2: polyphyllin Ⅵ; 3: polyphyllin Ⅱ; 4: polyphyllin Ⅰ)

    图  2  不同遮阴条件下七叶一枝花的丙二醛(MDA)含量变化

    不同小写字母代表同一指标下不同处理在P<0.05水平差异显著。Different lowercase letters indicate significant differences at P<0.05 level among different treatments.

    Figure  2.  Malondialdehyde (MDA) contents of Paris polyphylla Smith var. chinensis (Franch.) Hara under different shading treatments

    表  1  重楼皂苷标准品溶液线性回归方程

    Table  1.   HPLC regression equation of reference solution of four authentic saponins

    皂苷类型 Saponins type回归方程 Regression equationr线性范围 Linear range (μg)
    重楼皂苷Ⅰ Polyphyllin Ⅰy=418 655x−26 7260.99910.0398~3.9844
    重楼皂苷Ⅱ Polyphyllin Ⅱy=345 133x−14 4450.99940.0398~3.9884
    重楼皂苷Ⅵ Polyphyllin Ⅵy=487 983x−22 6550.99950.0399~3.994
    重楼皂苷Ⅶ Polyphyllin Ⅶy=318 393x−13 9750.99910.0399~3.9956
    下载: 导出CSV

    表  2  不同程度遮阴对七叶一枝花根茎中重楼皂苷含量的影响

    Table  2.   Saponins contents of Paris polyphylla Smith var. chinensis (Franch.) Hara under different shading treatments mg∙g−1 

    遮阴度
    Shading degree (%)
    重楼皂苷Ⅰ
    Polyphyllin Ⅰ
    重楼皂苷Ⅱ
    Polyphyllin Ⅱ
    重楼皂苷Ⅵ
    Polyphyllin Ⅵ
    重楼皂苷Ⅶ
    PolyphyllinⅦ
    重楼皂苷含量
    Saponins content
    701.06±0.21a1.25±0.40b1.48±0.24b3.79±0.54b
    801.45±0.28a2.52±0.09a3.22±0.11a7.19±0.42a
    901.11±0.01a0.23±0.06c0.53±0.07c1.87±0.13c
      同列不同小写字母表示不同遮阴度间在P<0.05水平差异显著。Different lowercase letters in the same column indicate significant differences at P<0.05 level among treatments.
    下载: 导出CSV

    表  3  不同遮阴处理下七叶一枝花的抗氧化酶活性变化

    Table  3.   Antioxidative enzymes activities of Paris polyphylla Smith var. chinensis (Franch.) Hara under different shading treatments U·g−1·min−1 

    遮阴度
    Shading degree (%)
    SOD活性
    SOD activity
    POD活性
    POD activity
    CAT活性
    CAT activity
    70123.38±4.99b3.20±0.06b21.43±1.01a
    80105.00±3.67c2.89±0.15c19.78±1.85a
    90165.09±10.94a4.90±0.09a5.74±0.97b
      同列不同小写字母表示不同遮阴度间在P<0.05水平差异显著。Different lowercase letters in the same column indicate significant differences at P<0.05 level among different treatments.
    下载: 导出CSV

    表  4  不同遮阴条件下七叶一枝花的光合色素含量变化

    Table  4.   Photosynthetic pigment contents of Paris polyphylla Smith var. chinensis (Franch.) Hara under different shading

    遮阴度
    Shading degree (%)
    叶绿素a
    Chlorophyll a
    (mg∙g−1)
    叶绿素b
    Chlorophyll b
    (mg∙g−1)
    类胡萝卜素
    Carotinoid
    (mg∙g−1)
    叶绿素a/b
    Chlorophyll a/b
    叶绿素总量
    Total chlorophyll
    (mg∙g−1)
    702.16±0.09c0.79±0.03b8.48±0.34b2.72±0.02b2.94±0.11c
    803.69±0.25a1.03±0.04a15.76±1.31a3.61±0.42a4.72±0.21a
    902.86±0.06b0.95±0.01a10.15±0.16b3.00±0.04b3.81±0.07b
      同列不同小写字母表示不同遮阴度间在P<0.05水平差异显著。Different lowercase letters in the same column indicate significant differences at P<0.05 level among different treatments.
    下载: 导出CSV

    表  5  不同遮阴处理下七叶一株花的可溶性物质含量变化

    Table  5.   Soluble substances contents of Paris polyphylla Smith var. chinensis (Franch.) Hara under different shading treatments mg∙g−1 

    遮阴度 Shading degree (%)可溶性糖 Soluble sugar可溶性蛋白质 Soluble protein游离氨基酸 Free amino acid脯氨酸 Proline
    70 0.97±0.01a 9.23±0.01b 4.83±0.02c 11.48±0.22a
    80 0.86±0.01b 11.28±0.05a 5.53±0.23b 8.88±0.47b
    90 0.28±0.01c 7.72±0.04c 7.54±0.61a 12.68±0.26a
      同列不同小写字母表示不同遮阴度间在P<0.05水平差异显著。Different lowercase letters in the same column indicate significant differences at P<0.05 level among different treatments.
    下载: 导出CSV

    表  6  七叶一枝花重楼皂苷含量及各生化指标的相关性分析

    Table  6.   Correlation analysis of polyphyllin content with biochemical indexes of Paris polyphylla Smith var. chinensis (Franch.)

    重楼皂苷
    Polyphyllin
    SODCATPODMDA叶绿素a
    Chlorophyll
    a
    叶绿素b
    Chlorophyll
    b
    叶绿素a/b
    Chlorophyll
    a/b
    叶绿素总量
    Total
    chlorophyll
    类胡萝卜素
    Carotinoid
    可溶性糖
    Soluble sugar
    可溶性蛋白质
    Soluble protein
    游离氨基酸
    Free amino acid
    脯氨酸
    Proline
    重楼皂苷
    Polyphyllin
    1.00 −0.90** 0.79* −0.89** −0.95** 0.46 −0.15 0.70* −0.02 −0.25 0.50 0.99** 0.03 −0.29
    SOD −0.90** 1.00 −0.90** 0.95** 0.90** −0.33 0.33 −0.45 0.18 0.50 −0.69* −0.91** 0.22 0.59
    CAT 0.79* −0.90** 1.00 −0.95** −0.74* 0.20 −0.59 0.50 −0.42 −0.54 0.89** 0.79* −0.54 −0.65
    POD −0.89** 0.95** −0.95** 1.00 0.88** −0.28 0.37 −0.59 0.18 0.41 −0.81** −0.89** 0.38 0.66
    MDA −0.95** 0.90** −0.74* 0.88** 1.00 −0.48 −0.03 −0.63 −0.16 0.13 −0.44 −0.97** −0.09 0.31
    叶绿素a
    Chlorophyll a
    0.46 −0.33 0.20 −0.28 −0.48 1.00 0.32 0.64 0.39 0.02 −0.08 0.50 0.40 0.21
    叶绿素b
    Chlorophyll b
    −0.15 0.33 −0.59 0.37 −0.03 0.32 1.00 0.09 0.96** 0.74* −0.72* −0.12 0.79* 0.53
    叶绿素a/b
    Chlorophyll a/b
    0.70* −0.45 0.50 −0.59 −0.63 0.64 0.09 1.00 0.25 0.16 0.33 0.67* 0.03 −0.02
    叶绿素总量
    Total chlorophyll
    −0.02 0.18 −0.42 0.18 −0.16 0.39 0.96** 0.25 1.00 0.66 −0.53 0.01 0.66 0.37
    类胡萝卜素
    Carotinoid
    −0.25 0.50 −0.54 0.41 0.13 0.02 0.74* 0.16 0.66 1.00 −0.52 −0.28 0.46 0.46
    可溶性糖
    Soluble sugar
    0.50 −0.69* 0.89** −0.81** −0.44 −0.08 −0.72* 0.33 −0.53 −0.52 1.00 0.47 −0.85** −0.87**
    可溶性蛋白质
    Soluble protein
    0.99** −0.91** 0.79* −0.89** −0.97** 0.50 −0.12 0.67* 0.01 −0.28 0.47 1.00 0.06 −0.27
    游离氨基酸
    Free amino acid
    0.03 0.22 −0.54 0.38 −0.09 0.40 0.79* 0.03 0.66 0.46 −0.85** 0.06 1.00 0.78*
    脯氨酸 Proline −0.29 0.59 −0.65 0.66 0.31 0.21 0.53 −0.02 0.37 0.46 −0.87** −0.27 0.78* 1.00
      **: P<0.01; *: P<0.05.
    下载: 导出CSV
  • [1] 国家药典委员会. 中华人民共和国药典[S]. 一部. 北京: 中国医药科技出版社, 2015: 260

    Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China[S]. Beijing: China Medical Science Press, 2015: 260
    [2] 丁立帅, 赵猛, 李燕敏, 等. 七叶一枝花根茎和地上部分提取物镇痛抗炎作用研究[J]. 天然产物研究与开发, 2018, 30(5): 832−839

    DING L S, ZHAO M, LI Y M, et al. Study on the anti-nociceptive and anti-inflammatory effects of the extract of aerial part and rhizome of Paris polyphylla var. chinensis[J]. Natural Product Research and Development, 2018, 30(5): 832−839
    [3] 杨远贵, 张霁, 张金渝, 等. 重楼属植物化学成分及药理活性研究进展[J]. 中草药, 2016, 47(18): 3301−3323 doi: 10.7501/j.issn.0253-2670.2016.18.027

    YANG Y G, ZHANG J, ZHANG J Y, et al. Research progress in chemical constituents in plants of Paris L. and their pharmacological effects[J]. Chinese Traditional and Herbal Drugs, 2016, 47(18): 3301−3323 doi: 10.7501/j.issn.0253-2670.2016.18.027
    [4] 闫道良, 田风光, 段承俐, 等. 浙江省七叶一枝花种质资源现状与表型多元统计分析[J]. 浙江林业科技, 2015, 35(3): 6−10

    YAN D L, TIAN F G, DUAN C L, et al. Investigation and phenotypic analysis on germplasm resources of Paris polyphylla var. chinensis in Zhejiang[J]. Journal of Zhejiang Forestry Science and Technology, 2015, 35(3): 6−10
    [5] 王璐, 李艳丽, 熊欢, 等. 温度胁迫对锥栗幼苗叶片结构及光合特性的影响[J]. 江西农业大学学报, 2020, 42(4): 692−699

    WANG L, LI Y L, XIONG H, et al. Effect of temperature stress on leaf structure and photosynthesis of chinquapin (Castanea henryi)[J]. Acta Agriculturae Universitatis Jiangxiensis, 2020, 42(4): 692−699
    [6] 张辉, 王荷, 张蓓蓓, 等. 光强对黑麦草萌发生长、叶片叶绿素含量及光系统Ⅱ的影响[J]. 干旱地区农业研究, 2018, 36(4): 207−213 doi: 10.7606/j.issn.1000-7601.2018.04.30

    ZHANG H, WANG H, ZHANG B B, et al. Effects of light intensity on germination, growth, chlorophyll content and photosystem Ⅱ of leaves in ryegrass (Lolium perenne L.)[J]. Agricultural Research in the Arid Areas, 2018, 36(4): 207−213 doi: 10.7606/j.issn.1000-7601.2018.04.30
    [7] 王宇, 刘洋, 刘佳, 等. 不同光照强度对黄芪主要次生代谢物含量的影响[J]. 应用与环境生物学报, 2017, 23(5): 928−933

    WANG Y, LIU Y, LIU J, et al. Effect of light intensity on the contents of main secondary metabolites in Astragalus[J]. Chinese Journal of Applied and Environmental Biology, 2017, 23(5): 928−933
    [8] 杨培芳, 史红专, 郭巧生, 等. 光照强度对紫花地丁物质和能量代谢的影响[J]. 中国中药杂志, 2020, 45(24): 5944−5950

    YANG P F, SHI H Z, GUO Q S, et al. Effects of light intensity on material and energy metabolism of Viola yedoensis[J]. China Journal of Chinese Materia Medica, 2020, 45(24): 5944−5950
    [9] ZUSHI K, SUEHARA C, SHIRAI M. Effect of light intensity and wavelengths on ascorbic acid content and the antioxidant system in tomato fruit grown in vitro[J]. Scientia Horticulturae, 2020, 274: 109673 doi: 10.1016/j.scienta.2020.109673
    [10] 贾兵, 范伟, 陈庚, 等. 建立UPLC-MS联合HPLC方法探究外源物质对三七叶皂苷含量和生长的影响[J]. 云南农业大学学报: 自然科学版, 2019, 34(1): 116−123

    JIA B, FAN W, CHEN G, et al. Development of UPLC-MS combined with HPLC technique to explore the effects of exogenous substances on the saponin contents and growth of Panax notoginseng leaves[J]. Journal of Yunnan Agricultural University: Natural Science, 2019, 34(1): 116−123
    [11] 焦晓林, 高微微. 环境因子对药用植物三萜皂苷合成影响的研究进展[J]. 中草药, 2011, 42(2): 398−402

    JIAO X L, GAO W W. Advances in studies on influence of environmental factors on triterpenoid saponin synthesis in medicinal plants[J]. Chinese Traditional and Herbal Drugs, 2011, 42(2): 398−402
    [12] 曾燕, 郭兰萍, 杨光, 等. 环境生态因子对药用植物皂苷成分的影响[J]. 中国实验方剂学杂志, 2012, 18(17): 313−318 doi: 10.3969/j.issn.1005-9903.2012.17.089

    ZENG Y, GUO L P, YANG G, et al. Effect of environmental ecological factors on saponins of medicinal plant[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(17): 313−318 doi: 10.3969/j.issn.1005-9903.2012.17.089
    [13] 郑顺林, 田孟良, 刘金亮, 等. 人工驯化栽培中弱光胁迫对七叶一枝花光合特性的影响[J]. 中药材, 2014, 37(9): 1518−1522

    ZHENG S L, TIAN M L, LIU J L, et al. Effects of Low-light stress on photosynthetic characteristics of Paris polyphylla var. chinensis in artificial domestication cultivation[J]. Journal of Chinese Medicinal Materials, 2014, 37(9): 1518−1522
    [14] 梁娟, 易涛, 叶漪. 遮阴对七叶一枝花光合特性及皂苷含量的影响[J]. 江苏农业科学, 2016, 44(4): 265−267

    LIANG J, YI T, YE Y. Effects of shading on photosynthetic characteristics and saponin contents of Paris polyphylla[J]. Jiangsu Agricultural Sciences, 2016, 44(4): 265−267
    [15] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000: 134, 258–260, 184

    LI H S. Principles and Techniques of Plant Physiological Biochemical Experiment[M]. Beijing: Higher Education Press, 2000: 134, 258–260, 184
    [16] 位杰, 吴翠云, 蒋媛, 等. 蒽酮法测定红枣可溶性糖含量条件的优化[J]. 食品科学, 2014, 35(24): 136−140 doi: 10.7506/spkx1002-6630-201424026

    WEI J, WU C Y, JIANG Y, et al. Sample preparation optimization for determination of soluble sugar in red jujube fruits by anthrone method[J]. Food Science, 2014, 35(24): 136−140 doi: 10.7506/spkx1002-6630-201424026
    [17] 国家药典委员会. 中华人民共和国药典[S]. 一部. 北京: 中国医药科技出版社, 2020: 276–277

    Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China[S]. Beijing: China Medical Science Press, 2020: 276–277
    [18] 匡双便, 徐祥增, 孟珍贵, 等. 不同透光率对三七生长特征及根皂苷含量的影响[J]. 应用与环境生物学报, 2015, 21(2): 279−286

    KUANG S B, XU X Z, MENG Z G, et al. Effects of light transmittance on plant growth and root ginsenoside content of Panax notoginseng[J]. Chinese Journal of Applied and Environmental Biology, 2015, 21(2): 279−286
    [19] 何忠俊, 黄希, 梁社往, 等. 滇重楼根茎皂苷含量与生态因子的关系[J]. 生态环境学报, 2016, 25(3): 409−414

    HE Z J, HUANG X, LIANG S W, et al. Relationships between total saponin content in rhizome of Paris polyphylla var. yunnannensis and ecological factors[J]. Ecology and Environmental Sciences, 2016, 25(3): 409−414
    [20] 吴长桥, 张睿胤, 穆德添, 等. 七叶一枝花糖基转移酶基因的克隆及表达分析[J]. 分子植物育种, 2021, 19(9): 2881−2888

    WU C Q, ZHANG R Y, MU D T, et al. Cloning and expression analysis of glycosyltransferase in Paris polyphylla Smith var. chinensis (Franch.) Hara[J]. Molecular Plant Breeding, 2021, 19(9): 2881−2888
    [21] LI A M, LI S H, WU X J, et al. Influence of light intensity on the yield and quality of Houttuynia cordata[J]. Plant Production Science, 2015, 18(4): 522−528 doi: 10.1626/pps.18.522
    [22] 郭品湘, 尹婷, 粟春青, 等. 遮阴对双色木番茄幼苗生理特性的影响[J]. 森林与环境学报, 2020, 40(1): 76−82

    GUO P X, YIN T, SU C Q, et al. Effects of shade on the physiological characteristics of Solanum wrightii seedlings[J]. Journal of Forest and Environment, 2020, 40(1): 76−82
    [23] 苏文华, 张光飞, 李秀华, 等. 植物药材次生代谢产物的积累与环境的关系[J]. 中草药, 2005, 36(9): 1415−1418 doi: 10.3321/j.issn:0253-2670.2005.09.052

    SU W H, ZHANG G F, LI X H, et al. Relationship between accumulation of secondary metabolism in medicinal plant and environmental condition[J]. Chinese Traditional and Herbal Drugs, 2005, 36(9): 1415−1418 doi: 10.3321/j.issn:0253-2670.2005.09.052
    [24] KOUKI M, MANETAS Y. Resource availability affects differentially the levels of gallotannins and condensed tannins in Ceratonia siliqua[J]. Biochemical Systematics and Ecology, 2002, 30(7): 631−639 doi: 10.1016/S0305-1978(01)00142-9
    [25] 李合生. 现代植物生理学[M]. 北京: 高等教育出版社, 2002

    LI H S. Modern Plant Physiology[M]. Beijing: Higher Education Press, 2002
    [26] 孙克香, 杨莎, 郭峰, 等. 高温强光胁迫下外源钙对甜椒(Capsicum fructescens L.)幼苗光合生理特性的影响[J]. 植物生理学报, 2015, 51(3): 280−286

    SUN K X, YANG S, GUO F, et al. Effects of exogenous calcium on photosynthetic chracteristics of sweet pepper (Capsicum fructescens L.) seedlings[J]. Plant Physiology Journal, 2015, 51(3): 280−286
    [27] 黄河腾, 黄剑坚, 陈杰, 等. 不同遮阴环境下木奶果幼苗生长与生理生化的响应[J]. 生态学杂志, 2020, 39(5): 1538−1547

    HUANG H T, HUANG J J, CHEN J, et al. Growth, physiological and biochemical response of Baccaurea ramiflora Lour. seedlings to different shading environments[J]. Chinese Journal of Ecology, 2020, 39(5): 1538−1547
    [28] 吴能表, 李琳琳, 杨卫星, 等. 光强对长春花叶片碳氮及次生代谢产物积累的影响[J]. 草业科学, 2014, 31(8): 1508−1514 doi: 10.11829/j.issn.1001-0629.2013-0584

    WU N B, LI L L, YANG W X, et al. Effects of light intensity on carbon-nitrogen metabolism and secondary metabolite of Catharanthus roseus leaves[J]. Pratacultural Science, 2014, 31(8): 1508−1514 doi: 10.11829/j.issn.1001-0629.2013-0584
    [29] 陶巧静, 吴月燕, 付涛, 等. 弱光胁迫对西洋杜鹃生理特性和叶片超微结构的影响[J]. 林业科学, 2015, 51(3): 84−92

    TAO Q J, WU Y Y, FU T, et al. Effect of low light stress on physiological characteristics and ultrastructure of Rhododendron hybridum leaves[J]. Scientia Silvae Sinicae, 2015, 51(3): 84−92
    [30] 徐明远, 王谦博, 郭盛磊, 等. 遮光对刺五加生长以及光合生理参数的影响[J]. 中国实验方剂学杂志, 2020, 26(20): 140−145

    XU M Y, WANG Q B, GUO S L, et al. Effect of shading on growth and photosynthetic physiological parameters of Acanthopanax senticosus[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2020, 26(20): 140−145
    [31] 严晓芦, 郭巧生, 史红专, 等. 光照强度对紫花地丁生长、生理及化学成分的影响[J]. 中国中药杂志, 2019, 44(6): 1119−1125

    YAN X L, GUO Q S, SHI H Z, et al. Effects of light intensities on growth, physiological characteristic and chemical composition of Viola yedoensis[J]. China Journal of Chinese Materia Medica, 2019, 44(6): 1119−1125
    [32] 季本华, 朱素琴, 焦德茂. 不同温光条件下籼粳稻叶片的光能转化效率和膜脂过氧化表现[J]. 作物学报, 2001, 27(6): 743−750

    JI B H, ZHU S Q, JIAO D M. Light energy conversion efficiency and membrane lipid peroxidation in leaves of indica and Japonica rice (Oryza sativa L.) under different temperatures and light intensities[J]. Acta Agronomica Sinica, 2001, 27(6): 743−750
    [33] 吕冬煦, 倪海瑞, 曾彦达. 光照强度对水稻HS叶片失绿的影响[J]. 中国农学通报, 2020, 36(20): 23−27

    LYU D X, NI H R, ZENG Y D. Light intensity: effects on leaf chlorosis of rice HS[J]. Chinese Agricultural Science Bulletin, 2020, 36(20): 23−27
  • 加载中
图(2) / 表(6)
计量
  • 文章访问数:  237
  • HTML全文浏览量:  67
  • PDF下载量:  33
  • 被引次数: 0
出版历程
  • 收稿日期:  2021-05-27
  • 录用日期:  2021-08-18
  • 网络出版日期:  2021-10-08
  • 刊出日期:  2022-01-08

目录

    /

    返回文章
    返回