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高温对冬小麦旗叶光合机构的伤害机制

杨程 李向东 杜思梦 张德奇 时艳华 王汉芳 邵运辉 方保停 程红建 位芳

杨程, 李向东, 杜思梦, 张德奇, 时艳华, 王汉芳, 邵运辉, 方保停, 程红建, 位芳. 高温对冬小麦旗叶光合机构的伤害机制[J]. 中国生态农业学报 (中英文), 2022, 30(3): 399−408 doi: 10.12357/cjea.20210469
引用本文: 杨程, 李向东, 杜思梦, 张德奇, 时艳华, 王汉芳, 邵运辉, 方保停, 程红建, 位芳. 高温对冬小麦旗叶光合机构的伤害机制[J]. 中国生态农业学报 (中英文), 2022, 30(3): 399−408 doi: 10.12357/cjea.20210469
YANG C, LI X D, DU S M, ZHANG D Q, SHI Y H, WANG H F, SHAO Y H, FANG B T, CHENG H J, WEI F. Photosystem damage mechanism in flag leaves of winter wheat under high temperature[J]. Chinese Journal of Eco-Agriculture, 2022, 30(3): 399−408 doi: 10.12357/cjea.20210469
Citation: YANG C, LI X D, DU S M, ZHANG D Q, SHI Y H, WANG H F, SHAO Y H, FANG B T, CHENG H J, WEI F. Photosystem damage mechanism in flag leaves of winter wheat under high temperature[J]. Chinese Journal of Eco-Agriculture, 2022, 30(3): 399−408 doi: 10.12357/cjea.20210469

高温对冬小麦旗叶光合机构的伤害机制

doi: 10.12357/cjea.20210469
基金项目: 河南省农业科学院优秀青年科技基金计划(2022YQ02)、国家自然科学基金项目(31601242)和河南省农业科学院基本科研业务费资助
详细信息
    作者简介:

    杨程, 主要研究方向为作物光合作用和逆境生理。E-mail: luckytiger.com@163.com

    通讯作者:

    李向东, 主要研究方向为小麦栽培和耕作制度。E-mail: hnlxd@126.com

  • 中图分类号: S184

Photosystem damage mechanism in flag leaves of winter wheat under high temperature

Funds: This study was supported by the Excellent Youth Fund of Henan Academy of Agricultural Sciences (2022YQ02), the National Natural Science Foundation of China (31601242) and the Basic Scientific Research Expenses of Henan Academy of Agricultural Sciences.
More Information
  • 摘要: 高温胁迫下小麦光合能力下降是影响小麦灌浆和导致产量下降的主要因素。探明高温对小麦旗叶光合机构的伤害机制有助于小麦高温抗性资源的合理利用。本研究选取35个河南省不同年代的主栽小麦品种, 在离体条件下采用快速叶绿素荧光、820 nm光反射、延迟荧光同步测定的方法, 对不同温度(22 ℃, 30 ℃, 38 ℃)处理下所有小麦品种光合电子传递活性相关参数进行了测定和分析。首先根据最大光化学效率(FV/FM)对高温的敏感程度将35个小麦品种分为高温不敏感(G1)和高温敏感(G2)两种类型, 然后对所测参数分别平均, 结果显示高温胁迫下两种类型小麦品种光系统Ⅱ (PSⅡ)的光能捕获效率、吸收的光能推动电子传递到初级醌受体(QA)下游和光系统Ⅰ (PSⅠ)末端的效率、质体醌(PQ)库的再还原能力高温下均显著下降, 但G2小麦品种下降幅度更大; 两种类型小麦品种PSⅠ活性均不受影响, 但G2小麦品种PSⅠ供体侧电子传递活性下降幅度大于G1小麦品种。通过3种研究方法的相互补充和印证, 最终表明PSⅡ反应中心活性、PSⅡ光能的捕获和QA向下游传递电子的能力的差异是导致两种类型小麦品种光合电子传递活性差异的主要原因, PSⅡ供体侧和PSⅠ活性对小麦光系统Ⅱ高温抗性没有直接影响。该研究对未来冬小麦抗高温品种选育和栽培管理技术的创新提供了参考。
  • 图  1  高温对高温不敏感型(G1)和高温敏感型(G2)小麦品种旗叶叶绿素荧光诱导曲线的影响(曲线已标准化)

    CK、T1和T2表示叶片处理温度分别为22 ℃、30 ℃和38 ℃。O、J、I和P均为荧光诱导动力学曲线的特征位点。CK, T1 and T2 are treatments of 22 ℃, 30 ℃ and 38 ℃. O, J, I, and P are characteristic sites of the fluorescence induction curve.

    Figure  1.  Effect of high temperature on chlorophyll fluorescence induction curves of wheat flag leaves of high-temperature insensitive (G1) and sensitive (G2) cultivars (the curve has been standardized)

    图  2  高温对高温不敏感型(G1)和高温敏感型(G2)小麦品种旗叶叶绿素荧光诱导曲线O-J段和O-K段荧光强度的影响

    图A和B通过公式VK=(FtFO)/(FJFO)对O-J段荧光标准化, 然后使用公式ΔVK=VKtreatmentVKCK进行二次标准化; 图C和D通过公式VL=(FtFO)/(FKFO)对O-K段荧光标准化, 然后使用公式ΔVL=VLtreatmentVLCK进行二次标准化。CK、T1和T2表示叶片处理温度分别为22 ℃、30 ℃和38 ℃。For fig. A and B, O-J segment were normalized by formula VK=(Ft FO)/(FJFO), then ΔVK was calculate by formula ΔVK=VKtreatmentVKCK. For fig. C and D, O-K segment were normalized by formula VL=(Ft FO)/(FKFO), then ΔVL was calculate by formula ΔVL=VLtreatmentVLCK. CK, T1 and T2 are treatments of 22 ℃, 30 ℃ and 38 ℃, respectively.

    Figure  2.  Effect of high temperature on the fluorescence intensities of O-J segment and O-K segment of chlorophyll fluorescence induction curves of wheat flag leaves of high-temperature insensitive (G1) and sensitive (G2) cultivars

    图  3  高温对高温不敏感型(G1)和高温敏感型(G2)小麦品种旗叶叶绿素荧光参数φPoφEoφRo、φDo、RC/CSmδRo、PIABS 的影响

    不同小写字母表示不同品种类型(G1和G2)不同温度间有显著差异(LSD, P<0.05), G1为13个小麦品种的平均值, G2为22个小麦品种的平均值。CK、T1和T2表示叶片处理温度分别为22 ℃、30 ℃和38 ℃。Different lowercase letters indicate significant differences among different temperatures of G1 and G2 (LSD, P<0.05). The value of G1 is the average of 13 wheat cultivars; the value G2 is the average of 22 wheat cultivars. CK, T1 and T2 are treatments of 22 ℃, 30 ℃ and 38 ℃, respectively.

    Figure  3.  Effect of high temperature on chlorophyll fluorescence parameters φPo, φEo, φRo, φDo, RC/CSm, δRo and PIABS of wheat flag leaves of high-temperature insensitive (G1) and sensitive (G2) cultivars

    图  4  高温对高温不敏感型(G1)和高温敏感型(G2)小麦品种旗叶820 nm光反射(MR rel.)及其最大下降速率(VPSⅠ)和上升速率(VPSⅡ−PSⅠ)的影响

    不同小写字母表示不同品种类型(G1和G2)不同处理间有显著差异(LSD, P<0.05), G1为13个小麦品种的平均值, G2为22个小麦品种的平均值。Different lowercase letters indicate significant differences among different temperatures of G1 and G2 (LSD, P<0.05). The value of G1 is the average of 13 wheat cultivars; G2 is the average of 22 wheat cultivars.

    Figure  4.  Effect of high temperature on the light reflection at MR820nm (MR rel.) and its maximum decrease rate (VPSⅠ) and rise rate (VPSⅡ−PSⅠ) of wheat flag leaves of high-temperature insensitive (G1) and sensitive (G2) cultivars

    图  5  高温对高温不敏感型(G1)和高温敏感型(G2)小麦品种旗叶延迟荧光诱导曲线(0.01~0.02 ms) (A和B)和延迟荧光参数I2/I1 (C)的影响

    不同小写字母表示不同品种类型(G1和G2)不同处理间有显著差异(LSD, P<0.05), G1为13个小麦品种的平均值, G2为22个小麦品种的平均值。I1和I2为延迟荧光诱导曲线的特征位点。Different letters indicate significant differences among different temperatures of G1 and G2 (LSD, P<0.05). The value of G1 is the average of 13 wheat cultivars; G2 is the average of 22 wheat cultivarss. I1 and I2 are characteristic sites of the delayed fluorescence induction curve.

    Figure  5.  Effect of high temperature on the delayed fluorescence induction curve (0.01−0.02 ms) (A and B) and delayed fluorescence parameter I2/I1 (C) of wheat flag leaves of high-temperature insensitive (G1) and sensitive (G2) cultivars

    表  1  选用的小麦品种

    Table  1.   Wheat cultivars used in this study

    编号
    Number
    品种名称
    Variety name
    编号
    Number
    品种名称
    Variety name
    1 碧玛1号 Bima 1 19 郑麦366 Zhengmai 366
    2 碧玛4号 Bima 4 20 周麦22 Zhoumai 22
    3 阿夫 Afu 21 郑麦7698 Zhengmai 7698
    4 阿勃 Abo 22 矮丰3号 Aifeng 3
    5 郑州7023 Zhengzhou 7023 23 郑麦0856 Zhengmai 0856
    6 丰产3号 Fengchan 3 24 郑麦0943 Zhengmai 0943
    7 郑州761 Zhengzhou 761 25 平原50 Pingyuan 50
    8 西安8号 Xi’an 8 26 葫芦头 Hulutou
    9 百农3217 Bainong 3217 27 大口麦 Dakoumai
    10 宝丰7228 Baofeng 7228 28 红和尚头 Hongheshangtou
    11 豫麦13 Yumai 13 29 西农6028 Xinong 6028
    12 周麦9号 Zhoumai 9 30 白玉皮 Baiyupi
    13 豫麦18 Yumai 18 31 偃展4110 Yanzhan 4110
    14 豫麦49 Yumai 49 32 内乡5号 Neixiang 5
    15 郑麦9023 Zhengmai 9023 33 博爱7023 Bo’ai 7023
    16 兰考矮早8 Lankaoaizao 8 34 宛7107 Wan 7107
    17 周麦18 Zhoumai 18 35 豫麦2号 Yumai2
    18 矮抗58 Aikang 58
    下载: 导出CSV

    表  2  叶绿素快速荧光诱导动力学曲线参数及公式

    Table  2.   Parameters and formulas of rapid chlorophyll fluorescence inducing kinetic curve

    参数
    Parameter
    计算方式
    Method of calculation
    FM暗适应后照光获得的最大荧光强度 Maximum fluorescence intensity obtained under light after dark adaptation
    FO叶绿素荧光诱导动力学曲线20 μs的荧光强度 Fluorescence intensity at 20 μs of rapid chlorophyll fluorescence inducing kinetic (JIP) curve
    FVFV=FM−FO
    Ftt时的荧光强度 Fluorescence intensity at t time
    FKK点(0.3 ms)的荧光强度 Fluorescence intensity at 0.3 ms
    FJJ点(3 ms)的荧光强度 Fluorescence intensity at 3 ms
    FII点(30 ms)的荧光强度 Fluorescence intensity at 30 ms
    VJVJ=(FJFO)/(FMFO)
    VIVI=(FIFO)/(FMFO)
    MOMO=4(F300μsFO)/(FMFO) F300μs为300 μs的荧光强度 F 300μs is fluorescence intensity at 300 μs.
    ΨOψO=1−VJ
    φPOφPO=Fv/FM=(FMFO)/FM
    φEOφEo=ETo/ABS=[1−(FO/FM)]×ψO
    φDOφDO=1−φPO
    φROφRO=φPO×(1−VI)
    σROσRO=(1−VI)/(1−VJ)
    ABS/RCABS/RC=MO×(1/VJ)×(1/φPO)
    RC/CSmRC/CSm=φPO×(VJ/MOFM
    PIABSPIABS=(RC/ABS)×[φPO/(1−φPO)]×[ψO/(1−ψO)]
    下载: 导出CSV

    表  3  不同类型小麦品种不同温度处理下的旗叶FV/FM及其38 ℃处理下的下降幅度和分类结果

    Table  3.   FV/FM of flag leaves at different temperatures and its decrease extents at 38 ℃, and classification results of different wheat cultivars

    品种类型
    Cultivar type
    品种编号
    Cultivar number
    22 ℃ (CK)30 ℃ (T1)38 ℃ (T2)38 ℃处理下降幅度
    Decline extent at 38 ℃ (%)
    G1 22 0.723±0.035 0.724±0.019 0.713±0.019 1.40
    G1 2 0.745±0.019 0.767±0.008 0.712±0.069 4.40
    G1 23 0.764±0.018 0.674±0.023 0.720±0.022 5.80
    G1 19 0.760±0.014 0.613±0.052 0.685±0.026 9.80
    G1 17 0.749±0.010 0.710±0.205 0.649±0.056 13.40
    G1 21 0.732±0.018 0.597±0.067 0.631±0.041 13.80
    G1 20 0.739±0.045 0.539±0.071 0.615±0.065 16.80
    G1 16 0.716±0.017 0.634±0.194 0.579±0.093 19.10
    G1 18 0.759±0.016 0.739±0.023 0.603±0.141 20.60
    G1 13 0.732±0.023 0.731±0.017 0.557±0.055 23.90
    G1 15 0.765±0.021 0.731±0.021 0.574±0.066 25.00
    G1 14 0.715±0.026 0.720±0.023 0.496±0.075 30.70
    G1 4 0.732±0.031 0.731±0.022 0.443±0.06 39.50
    G2 1 0.699±0.037 0.695±0.040 0.373±0.069 46.70
    G2 7 0.757±0.012 0.724±0.027 0.381±0.058 49.70
    G2 26 0.761±0.020 0.747±0.013 0.368±0.053 51.70
    G2 3 0.753±0.021 0.737±0.030 0.351±0.086 53.40
    G2 5 0.774±0.007 0.733±0.021 0.342±0.029 55.80
    G2 27 0.771±0.014 0.754±0.017 0.324±0.036 58.00
    G2 25 0.772±0.027 0.774±0.009 0.314±0.034 59.30
    G2 6 0.763±0.036 0.715±0.020 0.277±0.077 63.60
    G2 9 0.679±0.045 0.698±0.016 0.222±0.021 67.20
    G2 33 0.712±0.037 0.756±0.012 0.225±0.033 68.40
    G2 10 0.736±0.032 0.611±0.043 0.231±0.019 68.60
    G2 34 0.775±0.009 0.759±0.009 0.244±0.070 68.60
    G2 31 0.713±0.050 0.758±0.01 0.218±0.017 69.40
    G2 35 0.785±0.031 0.747±0.031 0.240±0.044 69.40
    G2 28 0.730±0.017 0.774±0.01 0.199±0.029 72.80
    G2 24 0.767±0.010 0.752±0.009 0.195±0.013 74.50
    G2 11 0.731±0.010 0.691±0.022 0.183±0.014 75.00
    G2 8 0.735±0.024 0.725±0.014 0.183±0.031 75.10
    G2 30 0.743±0.043 0.760±0.013 0.180±0.031 75.70
    G2 32 0.762±0.040 0.758±0.014 0.172±0.028 77.40
    G2 29 0.768±0.004 0.726±0.013 0.161±0.023 79.00
    G2 12 0.761±0.009 0.700±0.024 0.130±0.038 82.90
      G1为高温不敏感小麦类型, G2为高温敏感小麦类型。G1 represents the high temperature insensitive wheat cultivars and G2 represents the high temperature sensitive wheat cultivars.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-07-18
  • 录用日期:  2021-11-11
  • 网络出版日期:  2021-11-30
  • 刊出日期:  2022-03-07

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