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外源14-羟基芸苔素甾醇对冬小麦小花成粒及生理特性的影响

刘北城 段剑钊 戎亚思 张艳艳 贺利 王永华 郭天财 冯伟

刘北城, 段剑钊, 戎亚思, 张艳艳, 贺利, 王永华, 郭天财, 冯伟. 外源14-羟基芸苔素甾醇对冬小麦小花成粒及生理特性的影响[J]. 中国生态农业学报 (中英文), 2022, 30(7): 1143−1154 doi: 10.12357/cjea.20210622
引用本文: 刘北城, 段剑钊, 戎亚思, 张艳艳, 贺利, 王永华, 郭天财, 冯伟. 外源14-羟基芸苔素甾醇对冬小麦小花成粒及生理特性的影响[J]. 中国生态农业学报 (中英文), 2022, 30(7): 1143−1154 doi: 10.12357/cjea.20210622
LIU B C, DUAN J Z, RONG Y S, ZHANG Y Y, HE L, WANG Y H, GUO T C, FENG W. Effects of exogenous 14-hydroxybrassinolide on floret development into grain and physiological characteristics of winter wheat[J]. Chinese Journal of Eco-Agriculture, 2022, 30(7): 1143−1154 doi: 10.12357/cjea.20210622
Citation: LIU B C, DUAN J Z, RONG Y S, ZHANG Y Y, HE L, WANG Y H, GUO T C, FENG W. Effects of exogenous 14-hydroxybrassinolide on floret development into grain and physiological characteristics of winter wheat[J]. Chinese Journal of Eco-Agriculture, 2022, 30(7): 1143−1154 doi: 10.12357/cjea.20210622

外源14-羟基芸苔素甾醇对冬小麦小花成粒及生理特性的影响

doi: 10.12357/cjea.20210622
基金项目: 国家“十三五”重点研发计划项目(2017YFD0300204)、河南省科技攻关项目(212102110041)和财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-03)资助
详细信息
    作者简介:

    刘北城, 主要从事小麦栽培生理研究。E-mail: 18703825181@163.com

    通讯作者:

    冯伟, 主要从事小麦生理生态与生长监测研究。E-mail: fengwei78@126.com

  • 中图分类号: S512.1; S143.8; S311

Effects of exogenous 14-hydroxybrassinolide on floret development into grain and physiological characteristics of winter wheat

Funds: This study was supported by the National Key Research and Development Program of China (2017YFD0300204), the Key Technologies Research & Development Program of Henan Province (212102110041) and the Special Fund for Construction of China Agriculture Research System of Ministry of Finance and Ministry of Agriculture and Rural Affairs of the People’s Republic of China (CARS-03).
More Information
  • 摘要: 为探索14-羟基芸苔素甾醇(14-HBR)对小麦穗花发育成粒的影响, 以多穗型品种‘豫麦49-198’(V1)和大穗型品种‘周麦16’(V2)为材料, 在小麦拔节后20 d叶面喷施0.05 μmol∙L−1的14-HBR, 通过观察穗花发育进程, 测定旗叶碳氮代谢酶和不同器官碳氮含量及产量构成因素, 分析外源14-HBR对穗花发育的调控效应及促粒增产的生理机制。结果表明, 外源14-HBR可以有效增加小麦可孕小花数量, 提高可孕小花和小穗结实率, 对大穗型品种V2促进效果更明显。喷施14-HBR可以提高小麦旗叶Rubisco酶、蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS)以及谷氨酰胺合成酶(GS)和硝酸还原酶(NR)的活性, 且总体上对品种V2的促进效应更高。外源14-HBR可以提高穗和非穗器官的可溶性糖和氮含量及干物质积累量, 促进可溶性糖向穗部转运, 提高穗器官C/N比, 为穗花发育提供充足的碳营养, 且总体对品种V2的促进效果更好。考察V1和V2两品种产量及其构成因素发现, 喷施14-HBR对两品种穗数和千粒重影响较小, 而对穗粒数和产量促进效果显著, 其中产量增幅分别为9.31%和12.03%, 穗粒数增幅分别为9.85%和11.40%。喷施14-HBR主要通过提高小麦碳同化能力, 增强氮素吸收与积累, 促进碳营养物质向穗部转运和分配, 从而为穗花发育提供良好的物质基础以减少可孕小花的退化和败育, 进而提高小花结实数和结实率, 且对大穗型品种的影响更明显。
  • 图  1  外源喷施14-羟基芸苔素甾醇(14-HBR)对小麦多穗型品种‘豫麦49-198’(V1)和大穗型品种‘周麦16’(V2)穗花发育及结实的影响

    CK: 喷施清水对照; BR: 喷施14-HBR处理。不同小写字母表示不同处理间差异显著(P<0.05)。

    Figure  1.  Effect of exogenous spraying 14-hydroxybrassinolide (14-HBR) on spike and floret development and setting of multi-spike wheat cultivar ‘Yumai 49-198’ (V1) and large-spike wheat cultivar ‘Zhoumai 16’ (V2)

    CK: control with spraying water; BR: spraying 14-HBR. Different lowercase letters show significant differences among treatments (P<0.05).

    图  2  外源喷施14-羟基芸苔素甾醇(14-HBR)对小麦多穗型品种‘豫麦49-198’(V1)和大穗型品种‘周麦16’(V2)穗和非穗器官干物质积累的影响

    CK: 喷施清水对照; BR: 喷施14-HBR处理。

    Figure  2.  Effect of exogenous spraying 14-hydroxybrassinolide (14-HBR) on dry matter accumulation in spike and non-spike organs of multi-spike wheat cultivar ‘Yumai 49-198’ (V1) and large-spike wheat cultivar ‘Zhoumai 16’ (V2)

    CK: control with spraying water; BR: spraying 14-HBR.

    图  3  外源喷施14-羟基芸苔素甾醇(14-HBR)对小麦多穗型品种‘豫麦49-198’(V1)和大穗型品种‘周麦16’(V2)穗与非穗器官氮含量和氮积累量的影响

    CK: 喷施清水对照; BR: 喷施14-HBR处理。

    Figure  3.  Effects of exogenous spraying 14-hydroxybrassinolide (14-HBR) on nitrogen content and nitrogen accumulation in spike and non-spike organs of multi-spike wheat cultivar ‘Yumai 49-198’ (V1) and large-spike wheat cultivar ‘Zhoumai 16’ (V2)

    CK: control with spraying water; BR: spraying 14-HBR.

    图  4  外源喷施14-羟基芸苔素甾醇(14-HBR)对小麦多穗型品种‘豫麦49-198’(V1)和大穗型品种‘周麦16’(V2)穗和非穗器官可溶性糖含量的影响

    CK: 喷施清水对照; BR: 喷施14-HBR处理。CK: control with spraying water; BR: spraying 14-HBR.

    Figure  4.  Effect of exogenous spraying 14-hydroxybrassinolide (14-HBR) on soluble sugar content in spike and non-spike organs of multi-spike wheat cultivar ‘Yumai 49-198’ (V1) and large-spike wheat cultivar ‘Zhoumai 16’ (V2)

    图  5  外源喷施14-羟基芸苔素甾醇(14-HBR)对小麦多穗型品种‘豫麦49-198’(V1)和大穗型品种‘周麦16’(V2)穗和非穗器官C/N比的影响

    CK: 喷施清水对照; BR: 喷施14-HBR处理。CK: control with spraying water; BR: spraying 14-HBR.

    Figure  5.  Effect of exogenous spraying 14-hydroxybrassinolide (14-HBR) on C / N ratio in spike and non-spike organs of multi-spike wheat cultivar ‘Yumai 49-198’ (V1) and large-spike wheat cultivar ‘Zhoumai 16’ (V2)

    图  6  外源喷施14-羟基芸苔素甾醇(14-HBR)对小麦多穗型品种‘豫麦49-198’(V1)和大穗型品种‘周麦16’(V2)旗叶Rubisco、蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS)活性的影响

    CK: 喷施清水对照; BR: 喷施14-HBR处理。CK: control with spraying water; BR: spraying 14-HBR.

    Figure  6.  Effects of exogenous spraying 14-hydroxybrassinolide (14-HBR) on activities of Rubisco, sucrose phosphate synthase (SPS) and sucrose synthase (SS) enzymes in flag leaf of multi-spike wheat cultivar ‘Yumai 49-198’ (V1) and large-spike wheat cultivar ‘Zhoumai 16’ (V2)

    图  7  外源喷施14-羟基芸苔素甾醇(14-HBR)对小麦多穗型品种‘豫麦49-198’(V1)和大穗型品种‘周麦16’(V2)旗叶谷氨酰胺合成酶(GS)和硝酸还原酶(NR)活性的影响

    CK: 喷施清水对照; BR: 喷施14-HBR处理。CK: control with spraying water; BR: spraying 14-HBR.

    Figure  7.  Effect of exogenous spraying 14-hydroxybrassinolide (14-HBR) on activities of glutamine synthetase (GS) and nitrate reductase (NR) enzymes in flag leaf of multi-spike wheat cultivar ‘Yumai 49-198’ (V1) and large-spike wheat cultivar ‘Zhoumai 16’ (V2)

    表  1  外源喷施14- 羟基芸苔素甾醇(14-HBR)对不同小麦品种产量及其构成因素的影响

    Table  1.   Effect of exogenous spraying 14-hydroxybrassinolide (14-HBR) on yield and yield components of different wheat cultivars

    年度
    Year
    品种
    Cultivar
    处理
    Treatment
    穗数
    Spike number
    (×104spikes∙hm−2)
    穗粒数
    Grains number per spike
    千粒重
    1000-grain weight (g)
    产量
    Grain yield
    (kg∙hm−2)
    2018—2019豫麦49-198 Yumai 49-198CK612.87±2.29a33.66±0.76c40.41±0.68b7229.12±138.86d
    BR608.44±0.67a36.70±0.80b41.23±0.63b7968.66±37.90bc
    周麦16 Zhoumai 16CK520.86±2.19b37.01±1.04b44.26±2.13a8204.11±81.49b
    BR524.42±0.41b41.62±0.52a44.55±0.13a9143.48±87.81a
    2019—2020豫麦49-198 Yumai 49-198CK645.04±0.89a36.17±0.69c37.70±0.74bc7637.31±42.14c
    BR643.15±1.10a40.03±0.94b39.19±1.25b8278.03±31.27b
    周麦16 Zhoumai 16CK540.03±0.98b40.89±0.63b46.57±0.75a8308.42±100.77b
    BR542.46±1.24b45.12±0.72a47.85±0.21a9356.10±98.08a
    变异来源
    Source of variation
    品种 Cultivar********
    处理 TreatmentNS**NS**
    品种×处理
    Cultivar × treatment
    NSNSNS*
      ‘豫麦49-198’为多穗型品种, ‘周麦16’为大穗型品种。CK: 喷施清水; BR: 喷施14-HBR。同列数据后不同小写字母表示不同品种不同处理间差异显著(P<0.05)。NS: 差异不显著; *: P<0.05; **: P<0.01。‘Yumai49-198’ is a multi-spike wheat cultivar, and ‘Zhoumai16’ is a large-spike wheat cultivar. CK: control with spraying water; BR: spraying 14-HBR treatment. Different lowercase letters in the same column show significant differences among treatments of different cultivars (P<0.05). NS: no significant difference. * and ** indicate significant differences at P<0.05 and P<0.01, respectively.
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  • [1] 于振文, 田奇卓, 潘庆民, 等. 黄淮麦区冬小麦超高产栽培的理论与实践[J]. 作物学报, 2002, 28(5): 577−585 doi: 10.3321/j.issn:0496-3490.2002.05.001

    YU Z W, TIAN Q Z, PAN Q M, et al. Theory and practice on cultivation of super high yield of winter wheat in the wheat fields of Yellow River and Huaihe River districts[J]. Acta Agronomica Sinica, 2002, 28(5): 577−585 doi: 10.3321/j.issn:0496-3490.2002.05.001
    [2] 朱云集, 崔金梅, 郭天财, 等. 河南省小麦生产发展中几个关键技术问题的商榷[J]. 河南农业科学, 2011, 40(8): 54−57 doi: 10.3969/j.issn.1004-3268.2011.08.014

    ZHU Y J, CUI J M, GUO T C, et al. Discussion of key technique problems in development of wheat production in Henan Province[J]. Journal of Henan Agricultural Sciences, 2011, 40(8): 54−57 doi: 10.3969/j.issn.1004-3268.2011.08.014
    [3] DUAN J Z, WU Y P, ZHOU Y, et al. Grain number responses to pre-anthesis dry matter and nitrogen in improving wheat yield in the Huang-Huai Plain[J]. Scientific Reports, 2018, 8: 7126 doi: 10.1038/s41598-018-25608-0
    [4] 朱云集, 崔金梅, 王晨阳, 等. 小麦不同生育时期施氮对穗花发育和产量的影响[J]. 中国农业科学, 2002, 35(11): 1325−1329 doi: 10.3321/j.issn:0578-1752.2002.11.005

    ZHU Y J, CUI J M, WANG C Y, et al. Effects of nitrogen application at different wheat growth stages on floret development and grain yield of winter wheat[J]. Scientia Agricultura Sinica, 2002, 35(11): 1325−1329 doi: 10.3321/j.issn:0578-1752.2002.11.005
    [5] 朱元刚, 肖岩岩, 初金鹏, 等. 不同播期冬小麦小花发育特性与同化物代谢的相关性[J]. 植物营养与肥料学报, 2019, 25(3): 370−381 doi: 10.11674/zwyf.18119

    ZHU Y G, XIAO Y Y, CHU J P, et al. Correlation between floret development characteristics and photosynthate metabolism of winter wheat in different sowing dates[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(3): 370−381 doi: 10.11674/zwyf.18119
    [6] 朱云集, 郭天财, 王晨阳, 等. 两种穗型冬小麦品种产量形成特点及超高产关键栽培技术研究[J]. 麦类作物学报, 2006, 26(1): 82−86 doi: 10.3969/j.issn.1009-1041.2006.01.019

    ZHU Y J, GUO T C, WANG C Y, et al. Study on yield formation of winter wheat cultivars with different spike types and their key cultivation techniques for super-high yield[J]. Journal of Triticeae Crops, 2006, 26(1): 82−86 doi: 10.3969/j.issn.1009-1041.2006.01.019
    [7] ZHAO J F, WU C X, YUAN S J, et al. Kinase activity of OsBRI1 is essential for brassinosteroids to regulate rice growth and development[J]. Plant Science, 2013, 199/200: 113−120 doi: 10.1016/j.plantsci.2012.10.011
    [8] HAUBRICK L L, ASSMANN S M. Brassinosteroids and plant function: some clues, more puzzles[J]. Plant, Cell & Environment, 2006, 29(3): 446−457
    [9] CLOUSE S D. Molecular genetic studies confirm the role of brassinosteroids in plant growth and development[J]. The Plant Journal, 1996, 10(1): 1−8 doi: 10.1046/j.1365-313X.1996.10010001.x
    [10] FEDINA E O, KARIMOVA F G, TARCHEVSKY I A, et al. Effect of epibrassinolide on tyrosine phosphorylation of the Calvin cycle enzymes[J]. Russian Journal of Plant Physiology, 2008, 55(2): 193−200 doi: 10.1134/S1021443708020052
    [11] EPHRITIKHINE G, FELLNER M, VANNINI C, et al. The sax1 dwarf mutant of Arabidopsis thaliana shows altered sensitivity of growth responses to abscisic acid, auxin, gibberellins and ethylene and is partially rescued by exogenous brassinosteroid[J]. The Plant Journal, 1999, 18(3): 303−314 doi: 10.1046/j.1365-313X.1999.00454.x
    [12] PETZOLD U, PESCHEL S, DAHSE I, et al. Stimulation of source-applied 14C-sucrose export in Vicia faba plants by brassinosteroids, GA3 and IAA[J]. Acta Botanica Neerlandica, 1992, 41(4): 469−479 doi: 10.1111/j.1438-8677.1992.tb00517.x
    [13] 王学奎, 骆炳山, 屈映兰. 油菜素内酯促进小麦灌浆结实及其生理基础的研究[J]. 华中农业大学学报, 1990, 9(2): 123−128

    WANG X K, LUO B S, QU Y L. The promotion on the milking and formation of grains in wheat and its biological mechanism[J]. Journal of Huazhong Agricultural, 1990, 9(2): 123−128
    [14] SAIRAM R K. Effects of homobrassinolide application on plant metabolism and grain yield under irrigated and moisture-stress conditions of two wheat varieties[J]. Plant Growth Regulation, 1994, 14(2): 173−181 doi: 10.1007/BF00025220
    [15] THOMPSON M J, MANDAVA N B, MEUDT W J, et al. Synthesis and biological activity of brassinolide and its 22β, 23β-isomer: Novel plant growth-promoting steroids[J]. Steroids, 1981, 38(5): 567−580 doi: 10.1016/0039-128X(81)90055-6
    [16] BACK T G, PHARIS R P. Structure-activity studies of brassinosteroids and the search for novel analogues and mimetics with improved bioactivity[J]. Journal of Plant Growth Regulation, 2003, 22(4): 350−361 doi: 10.1007/s00344-003-0057-0
    [17] 王强锋, 李芹, 夏中梅, 等. 芸苔素类物质生物学活性比较研究与评价[J]. 西南农业学报, 2020, 33(12): 2766−2774

    WANG Q F, LI Q, XIA Z M, et al. Comparation and evaluation of biological activities of brassins[J]. Southwest China Journal of Agricultural Sciences, 2020, 33(12): 2766−2774
    [18] 崔金梅, 郭天财, 朱云集, 等. 小麦的穗[M]. 北京: 中国农业出版社, 2008

    CUI J M, GUO T C, ZHU Y J, et al. Spike of Wheat[M]. Beijing: China Agriculture Press, 2008
    [19] 张志良, 瞿伟菁, 李小方. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2009

    ZHANG Z L, QU W J, LI X F. Experimental Guide for Plant Physiology[M]. Beijing: Higher Education Press, 2009
    [20] MIRALLES D J, KATZ S D, COLLOCA A, et al. Floret development in near isogenic wheat lines differing in plant height[J]. Field Crops Research, 1998, 59(1): 21−30 doi: 10.1016/S0378-4290(98)00103-8
    [21] FISCHER R A. The importance of grain or kernel number in wheat: a reply to Sinclair and Jamieson[J]. Field Crops Research, 2008, 105(1/2): 15−21
    [22] LI S N, SONG M, DUAN J Z, et al. Regulation of spraying 6-BA in the late jointing stage on the fertile floret development and grain setting in winter wheat[J]. Agronomy, 2019, 9(9): 546 doi: 10.3390/agronomy9090546
    [23] PRYSTUPA P, SAVIN R, SLAFER G A. Grain number and its relationship with dry matter, N and P in the spikes at heading in response to N × P fertilization in barley[J]. Field Crops Research, 2004, 90(2/3): 245−254
    [24] GHIGLIONE H O, GONZALEZ F G, SERRAGO R, et al. Autophagy regulated by day length determines the number of fertile florets in wheat[J]. The Plant Journal, 2008, 55(6): 1010−1024 doi: 10.1111/j.1365-313X.2008.03570.x
    [25] 王志敏, 王树安, 苏宝林. 小麦穗粒数的调节Ⅱ: 开花前遮光对穗碳水化合物代谢和内源激素水平的影响[J]. 华北农学报, 1997, 12(4): 42−47 doi: 10.3321/j.issn:1000-7091.1997.04.009

    WANG Z M, WANG S A, SU B L. Regulation of grain number in wheat Ⅱ: Effects of shading on carbohydrate metabolism and hormone levels in spikes before anthesis[J]. Acta Agriculturae Boreali-Sinica, 1997, 12(4): 42−47 doi: 10.3321/j.issn:1000-7091.1997.04.009
    [26] SCHILLING G, SCHILLER C, OTTO S. Influence of brassinosteroids on organ relations and enzyme activities of sugar-beet plants[M]//ACS Symposium Series. Washington, DC: American Chemical Society, 1991: 208–219
    [27] GOETZ M, GODT D E, ROITSCH T. Tissue-specific induction of the mRNA for an extracellular invertase isoenzyme of tomato by brassinosteroids suggests a role for steroid hormones in assimilate partitioning[J]. The Plant Journal, 2000, 22(6): 515−522 doi: 10.1046/j.1365-313x.2000.00766.x
    [28] FARIDUDDIN Q, YUSUF M, HAYAT S, et al. Effect of 28-homobrassinolide on antioxidant capacity and photosynthesis in Brassica juncea plants exposed to different levels of copper[J]. Environmental and Experimental Botany, 2009, 66(3): 418−424 doi: 10.1016/j.envexpbot.2009.05.001
    [29] KANDELINSKAYA O L, TOPUNOV A F, GRISHCHENKO E R. Biochemical aspects of growth-stimulating effects of steroid phytohormones on lupine plants[J]. Applied Biochemistry and Microbiology, 2007, 43(3): 324−331 doi: 10.1134/S0003683807030155
    [30] 李勇, 王红光, 李瑞奇, 等. 表油菜素内酯对冬小麦产量及氮素吸收、积累和分配的影响[J]. 麦类作物学报, 2015, 35(2): 239−250 doi: 10.7606/j.issn.1009-1041.2015.02.013

    LI Y, WANG H G, LI R Q, et al. Effect of epibrassinolide on grain yield, and absorption, accumulation and distribution of nitrogen in winter wheat[J]. Journal of Triticeae Crops, 2015, 35(2): 239−250 doi: 10.7606/j.issn.1009-1041.2015.02.013
    [31] 刘捷平, 邱泽生, 王沅, 等. 冬小麦小花分化和退化及其与碳氮代谢的关系[J]. 北京师院学报: 自然科学版, 1980, 1(00): 84−89

    LIU J P, QIU Z S, WANG Y, et al. Flower differentiation and degradation of winter wheat and its relationship with carbon and nitrogen metabolism[J]. Journal of Beijing Normal University: Natural Science, 1980, 1(00): 84−89
    [32] 邱泽生, 刘捷平, 黄勤妮, 等. 冬小麦的小花发育与碳氮代谢的关系[J]. 作物学报, 1980, 6(3): 139−146

    QIU Z S, LIU J P, HUANG Q N, et al. The relation between floret development and carbonnitrogen metabolism in winter wheat[J]. Acta Agronomica Sinica, 1980, 6(3): 139−146
    [33] 于振文. 不同密度条件对冬小麦小花发育的影响[J]. 作物学报, 1984, 10(3): 185−194

    YU Z W. Influence of different density conditions on floret development of winter wheat[J]. Acta Agronomica Sinica, 1984, 10(3): 185−194
    [34] FRANK A B, BAUER A. Temperature, nitrogen, and carbon dioxide effects on spring wheat development and spikelet numbers[J]. Crop Science, 1996, 36(3): 659−665 doi: 10.2135/cropsci1996.0011183X003600030024x
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出版历程
  • 收稿日期:  2021-09-11
  • 录用日期:  2022-01-06
  • 网络出版日期:  2022-02-17
  • 刊出日期:  2022-07-05

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