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不同时期接种黄孢原毛平革菌对稻壳和鸡粪堆肥腐殖化的影响

马丽婷 徐智 赵兵 陈妍 邓亚琴 王宇蕴

马丽婷, 徐智, 赵兵, 陈妍, 邓亚琴, 王宇蕴. 不同时期接种黄孢原毛平革菌对稻壳和鸡粪堆肥腐殖化的影响[J]. 中国生态农业学报 (中英文), 2022, 30(9): 1522−1530 doi: 10.12357/cjea.20210903
引用本文: 马丽婷, 徐智, 赵兵, 陈妍, 邓亚琴, 王宇蕴. 不同时期接种黄孢原毛平革菌对稻壳和鸡粪堆肥腐殖化的影响[J]. 中国生态农业学报 (中英文), 2022, 30(9): 1522−1530 doi: 10.12357/cjea.20210903
MA L T, XU Z, ZHAO B, CHEN Y, DENG Y Q, WANG Y Y. Effect of Phanerochaete chrysosporium inoculation in different times of rice husk and chicken manure composting on humification of compost[J]. Chinese Journal of Eco-Agriculture, 2022, 30(9): 1522−1530 doi: 10.12357/cjea.20210903
Citation: MA L T, XU Z, ZHAO B, CHEN Y, DENG Y Q, WANG Y Y. Effect of Phanerochaete chrysosporium inoculation in different times of rice husk and chicken manure composting on humification of compost[J]. Chinese Journal of Eco-Agriculture, 2022, 30(9): 1522−1530 doi: 10.12357/cjea.20210903

不同时期接种黄孢原毛平革菌对稻壳和鸡粪堆肥腐殖化的影响

doi: 10.12357/cjea.20210903
基金项目: 国家自然科学基金项目(31760609)、中央引导高校发展杰出人才专项(A3012020017013)和云南省高层次人才培养支持计划青年拔尖人才专项(A3012020057)资助
详细信息
    作者简介:

    马丽婷, 主要研究方向为固体废弃物资源化利用。E-mail: 3294373755@qq.com

    通讯作者:

    王宇蕴, 主要研究方向为养分循环利用。E-mail: yuyunwhere@163.com

  • 中图分类号: X712

Effect of Phanerochaete chrysosporium inoculation in different times of rice husk and chicken manure composting on humification of compost

Funds: The study was supported by the National Natural Science Foundation of China (31760609), the Special Project for the Development of Outstanding Talents in Universities by the Central Government of China (A3012020017013) and the Top Young Talents of Yunnan High-level Talent Training and Support Program (A3012020057).
More Information
  • 摘要: 为了实现稻壳和鸡粪堆肥中木质纤维素的高效降解, 促进堆肥的腐殖化, 采用黄孢原毛平革菌(Phanerochaete chrysosporium)作为接种剂, 设置T1 (不接菌)、T2 [堆肥降温期(第18天)接菌]和T3 [堆肥初期(第0 天)接菌] 3个处理, 通过研究在不同堆肥时期接种黄孢原毛平革菌强化木质素和纤维素降解的作用, 关键酶活性变化及其与腐质化过程的偶联, 以确定接种黄孢原毛平革菌对堆肥腐殖化的促进作用。结果表明, 与T1处理相比, T2处理的木质素过氧化物酶和锰过氧化物酶活性、木质素和纤维素降解率和腐殖化程度均显著提高(P<0.05)。堆肥结束时, 与T1相比, T2的木质素和纤维素含量显著降低78.57%和52.63% (P<0.05), 与T3相比, T2木质素和纤维素含量显著降低57.14%和40.00% (P<0.05); T2腐殖质含量分别比T1和T3显著提高67.84%和52.33% (P<0.05)。此外, 多种腐熟指标表明T2处理的堆肥品质显著提高, 其腐殖化率、胡富比和胡敏酸百分比分别为51.64%、2.72和73.39%, 显著高于T1和T3处理(P<0.05)。RDA和相关性分析结果表明, 木质素和纤维素的降解与堆肥腐殖化指标呈显著负相关性(P<0.05), 木质过氧化物酶和锰过氧化物酶活性与木质素和纤维素含量呈显著负相关(P<0.05)。因此, 在堆肥降温期接种黄孢原毛平革菌通过提高木质过氧化物酶和锰过氧化物酶活性, 促进了木质素和纤维素的深度降解, 从而强化堆肥的腐殖化过程, 提高了堆肥品质。
  • 图  1  不同时间接种黄孢原毛平革菌对堆肥过程中堆肥物料理化性质的影响

    T1: 不接菌; T2: 堆肥第18天接菌; T3: 堆肥第0天接菌。

    Figure  1.  Changes of physicochemical properties of compost materials during composting with inoculation of Phanerochaete chrysosporium in different times

    T1: no inoculation; T2: inoculation on the day 18; T3: inoculation on the day 0.

    图  2  不同时间接种黄孢原毛平革菌对堆肥过程中堆肥物料酶活性的影响

    T1: 不接菌; T2: 堆肥第18天接菌; T3: 堆肥第0天接菌。

    Figure  2.  Changes of enzymes activities of compost materials during composting with inoculation of Phanerochaete chrysosporium in different times

    T1: no inoculation; T2: inoculation on the day 18; T3: inoculation on the day 0.

    图  3  不同时间接种黄孢原毛平革菌对堆肥过程中堆肥物料纤维素含量、木质素含量和有机物料降解率的影响

    T1: 不接菌; T2: 堆肥第18天接菌; T3: 堆肥第0天接菌。

    Figure  3.  Changes of cellulose content, lignin content and organic material degradation rate of compost materials during composting with inoculation of Phanerochaete chrysosporium in different times

    T1: no inoculation; T2: inoculation on the day 18; T3: inoculation on the day 0.

    图  4  不同时间接种黄孢原毛平革菌对堆肥过程中堆肥物料腐殖质组分和腐熟指标的影响

    T1: 不接菌; T2: 堆肥第18天接菌; T3: 堆肥第0天接菌。

    Figure  4.  Changes of humus components contents and maturity indexes of compost materials during composting with inoculation of Phanerochaete chrysosporium in different times

    T1: no inoculation; T2: inoculation on the day 18; T3: inoculation on the day 0.

    图  5  木质纤维素含量与木质纤维素降解酶活性之间的相关性(a)及堆肥腐熟程度和理化参数之间的冗余分析(b)

    *: P<0.05; **: P<0.01. LiP: 木质素过氧化物酶活性; MnP: 锰过氧化物酶活性; CC: 纤维素含量; LC: 木质素含量; GI: 种子发芽指数; DR: 有机物料降解率; HS: 腐殖质含量; HA: 胡敏酸含量; FA: 富里酸含量; HR: 腐殖化率; HA/FA: 胡富比; PHA: 胡敏酸百分比; Temperature: 温度。

    Figure  5.  Correlation between lignocellulose content and lignocellulose-degrading enzymes activities (a), and redundancy analysis between compost maturity indexes and compost physicochemical parameters (b)

    LiP: lignin peroxidase activity; MnP: manganese peroxidase activity; CC: cellulose content; LC: lignin content; GI: seed germination index; DR: organic material degradation rate; HS: humus content; HA: humic acid content; FA: fulvic acid content; HR: humification rate; HA/FA: ratio of humic acid to fulvic acid; PHA: percentage of humic acid.

    表  1  堆肥原料的基本理化性质

    Table  1.   Basic physicochemical properties of the compost raw materials

    原料
    Material
    总有机碳
    Total organic carbon (g∙kg−1)
    总氮
    Total nitrogen (g∙kg−1)
    水分
    Moisture (%)
    C/N
    鸡粪 Chicken manure329.7233.0555.419.98
    稻壳 Rice husk448.755.399.7983.26
    下载: 导出CSV
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