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蓝、紫单色光处理对三叶青生理及总黄酮含量时空变化的影响

刘守赞 蒋玲苔 张韵 顾依雯 韩敏琪 李姗 王红珍 白岩

刘守赞, 蒋玲苔, 张韵, 顾依雯, 韩敏琪, 李姗, 王红珍, 白岩. 蓝、紫单色光处理对三叶青生理及总黄酮含量时空变化的影响[J]. 中国生态农业学报 (中英文), 2023, 31(6): 885−894 doi: 10.12357/cjea.20230118
引用本文: 刘守赞, 蒋玲苔, 张韵, 顾依雯, 韩敏琪, 李姗, 王红珍, 白岩. 蓝、紫单色光处理对三叶青生理及总黄酮含量时空变化的影响[J]. 中国生态农业学报 (中英文), 2023, 31(6): 885−894 doi: 10.12357/cjea.20230118
LIU S Z, JIANG L T, ZHANG Y, GU Y W, HAN M Q, LI S, WANG H Z, BAI Y. Spatiotemporal changes in physiology and total flavonoids of Tetrastigma hemsleyanum in response to blue and purple monochromatic light[J]. Chinese Journal of Eco-Agriculture, 2023, 31(6): 885−894 doi: 10.12357/cjea.20230118
Citation: LIU S Z, JIANG L T, ZHANG Y, GU Y W, HAN M Q, LI S, WANG H Z, BAI Y. Spatiotemporal changes in physiology and total flavonoids of Tetrastigma hemsleyanum in response to blue and purple monochromatic light[J]. Chinese Journal of Eco-Agriculture, 2023, 31(6): 885−894 doi: 10.12357/cjea.20230118

蓝、紫单色光处理对三叶青生理及总黄酮含量时空变化的影响

doi: 10.12357/cjea.20230118
基金项目: 浙江省尖兵领雁研发攻关计划项目(2023C02054)、浙江农林大学校发展基金(2022LFR111)和中央财政林业科技推广示范资金项目(2019TS08)资助
详细信息
    作者简介:

    刘守赞, 研究方向为中药资源的收集、保护与开发, E-mail: szliu@zafu.edu.cn

    蒋玲苔, 研究方向为药用植物次生代谢调控, E-mail: 2021621032015@stu.zafu.edu.cn

    通讯作者:

    白岩, 研究方向为药用植物品质调控和中药资源挖掘整理, E-mail: hzbaiyan@zafu.edu.cn

    王红珍, 研究方向为药用植物次生代谢调控, E-mail: wang.hongzhen@163.com

  • 中图分类号: R932

Spatiotemporal changes in physiology and total flavonoids of Tetrastigma hemsleyanum in response to blue and purple monochromatic light

Funds: This study was supported by the Key Research and Development Project of “Vanguard” and “Leading Goose” in Zhejiang Province (2023C02054), Zhejiang Agriculture and Forestry University Development Fund (2022LFR111), and the Forestry Science and Technology Promotion Demonstration Fund Project of Central Finance of China (2019TS08).
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  • 摘要: 三叶青作为“新浙八味”之一的药材, 具有抗肿瘤、抗病毒和退热等临床效果, 其主要有效成分为黄酮类化合物。三叶青生长在林间荫蔽环境, 其活性成分合成、生长发育、生理变化等均会受到林下短波光调控。为明晰短波光对三叶青品质及生理变化的调控规律, 本文选择了蓝光和紫光单色光作为处理光源, 以白光作为对照, 分别处理7 d、15 d、30 d、45 d和60 d, 测定三叶青总黄酮含量、黄酮代谢合成酶活性、生理生化指标(光合色素、抗逆酶活性和丙二醛含量、初生代谢可溶物), 以期探讨三叶青黄酮代谢和生理生化变化对短波光条件的响应及规律。结果表明: 持续的单色短波光处理, 使三叶青处于轻度胁迫状态, 蓝光处理后三叶青中总黄酮含量最高, 其中叶片含量为137.75 mg∙g−1, 块根中为149.00 mg∙g−1; 紫光处理使叶片和块根中的苯丙氨酸酶活性[96.50 U∙g−1(FW)、109.47 U∙g−1(FW)]和叶片中查尔酮异构酶活性[65.17 U∙g−1(FW)]均达最高, 蓝光处理块根中的查尔酮合成酶活性最高, 为46.76 U∙g−1(FW)。此外, 光合色素、抗逆能力和可溶性物质对短波光的响应均有差异, 与白光相比, 蓝光和紫光均可促进渗透调节物质的积累。本研究的结果可为光调控三叶青品质提供理论基础和实践指导。
  • 图  1  蓝光和紫光对三叶青光合色素的影响

    不同小写字母表示同一处理时间不同处理间P<0.05水平差异显著。Different lowercase letters indicate significant differences among treatments in the same time at P<0.05 level.

    Figure  1.  Effects of blue and purple light on pigment contents of Tetrastigma hemsleyanum

    图  2  蓝光和紫光对三叶青叶片保护酶活性和丙二醛含量的影响

    不同小写字母表示在P<0.05水平差异显著。Different lowercase letters indicate significant differences at P<0.05 level.

    Figure  2.  Effects of blue and purple light on the activities of protective enzymes and malondialdehyde content in leaves of Tetrastigma hemsleyanum

    图  3  蓝光和紫光处理对三叶青渗透调节物质的影响

    不同小写字母表示在P<0.05水平差异显著。Different lowercase letters indicate significant differences at P<0.05 level.

    Figure  3.  Effects of blue and purple light treatments on osmotic adjustment substances of Tetrastigma hemsleyanum

    图  4  蓝光和紫光对三叶青黄酮合成酶活性的影响

    不同小写字母表示在P<0.05水平差异显著。

    Figure  4.  Effects of blue and purple light treatments on activities of flavonoids synthases of Tetrastigma hemsleyanum

    Different lowercase letters indicate significant differences at P<0.05 level. PAL: phenylalaninase activity; CHS: Charles ketone synthetase activity; CHI: Charles ketone isomerase activity.

    图  5  蓝光和紫光对三叶青总黄酮含量的影响

    不同小写字母表示在P<0.05水平差异显著。Different lowercase letters indicate significant differences at P<0.05 level.

    Figure  5.  Effects of blue and purple light treatments on contents of total flavonoids of Tetrastigma hemsleyanum

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出版历程
  • 收稿日期:  2023-03-03
  • 录用日期:  2023-03-28
  • 网络出版日期:  2023-03-17
  • 刊出日期:  2023-06-10

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