Spatiotemporal changes in physiology and total flavonoids of Tetrastigma hemsleyanum in response to blue and purple monochromatic light
-
摘要: 三叶青作为“新浙八味”之一的药材, 具有抗肿瘤、抗病毒和退热等临床效果, 其主要有效成分为黄酮类化合物。三叶青生长在林间荫蔽环境, 其活性成分合成、生长发育、生理变化等均会受到林下短波光调控。为明晰短波光对三叶青品质及生理变化的调控规律, 本文选择了蓝光和紫光单色光作为处理光源, 以白光作为对照, 分别处理7 d、15 d、30 d、45 d和60 d, 测定三叶青总黄酮含量、黄酮代谢合成酶活性、生理生化指标(光合色素、抗逆酶活性和丙二醛含量、初生代谢可溶物), 以期探讨三叶青黄酮代谢和生理生化变化对短波光条件的响应及规律。结果表明: 持续的单色短波光处理, 使三叶青处于轻度胁迫状态, 蓝光处理后三叶青中总黄酮含量最高, 其中叶片含量为137.75 mg∙g−1, 块根中为149.00 mg∙g−1; 紫光处理使叶片和块根中的苯丙氨酸酶活性[96.50 U∙g−1(FW)、109.47 U∙g−1(FW)]和叶片中查尔酮异构酶活性[65.17 U∙g−1(FW)]均达最高, 蓝光处理块根中的查尔酮合成酶活性最高, 为46.76 U∙g−1(FW)。此外, 光合色素、抗逆能力和可溶性物质对短波光的响应均有差异, 与白光相比, 蓝光和紫光均可促进渗透调节物质的积累。本研究的结果可为光调控三叶青品质提供理论基础和实践指导。Abstract: Tetrastigma hemsleyanum Diels et Gilg has antitumor, antiviral, and fever-relieving properties. The main active components are flavonoids. It grows in shaded environments in forests, and its growth, development, physiological changes, and synthesis of active components are regulated by short-wavelength light of undergrowth. To explore the spatial and temporal responses of physiological mechanisms and flavonoid synthesis in T. hemsleyanum to short-wavelength light, blue or purple monochromatic light were selected as treatment light sources, and white light was used as the control for 7, 15, 30, 45, and 60 d. The total flavonoids content, flavonoid-related enzymes activities, and physiological and biochemical indexes, such as photosynthetic pigments, anti-resistance enzymes activities, malondialdehyde (MDA), and primary metabolic solubility, of leaves were measured to investigate the spatial and temporal responses of the flavonoid synthesis process and physiological and biochemical changes in T. hemsleyanum to short-wavelength light conditions. The results showed that: 1) short-wavelength light treatments regulated the changes in photosynthetic pigments contents; the control and blue light treatments increased photosynthetic pigments contents, but the purple light treatment significantly inhibited this effect. 2) Continuous monochromatic short-wave light treatments put T. hemsleyanum under mild stress, and the total flavonoids content was the highest with blue light treatment (137.75 mg∙g−1 in leaves and 149.00 mg∙g−1 in tubers). The phenylalnine ammonialyase activities in leaves and tubers (96.50 U∙g−1 FW, 109.47 U∙g−1 FW) and chalcone isomerase activity in leaves (65.17 U∙g−1 FW) were highest under purple light treatment; while chalcone synthase activity in tubers was the highest with blue light treatment (46.76 U∙g−1 FW). In addition, photosynthetic pigments, resilience, and soluble substances differed in their responsiveness to short-wave light, and both blue and purple light promoted the accumulation of osmoregulatory substances compared with white light. This study provided a theoretical basis and practical guidance for light regulation of T. hemsleyanum quality.
-
图 1 蓝光和紫光对三叶青光合色素的影响
不同小写字母表示同一处理时间不同处理间P<0.05水平差异显著。Different lowercase letters indicate significant differences among treatments in the same time at P<0.05 level.
Figure 1. Effects of blue and purple light on pigment contents of Tetrastigma hemsleyanum
图 2 蓝光和紫光对三叶青叶片保护酶活性和丙二醛含量的影响
不同小写字母表示在P<0.05水平差异显著。Different lowercase letters indicate significant differences at P<0.05 level.
Figure 2. Effects of blue and purple light on the activities of protective enzymes and malondialdehyde content in leaves of Tetrastigma hemsleyanum
图 3 蓝光和紫光处理对三叶青渗透调节物质的影响
不同小写字母表示在P<0.05水平差异显著。Different lowercase letters indicate significant differences at P<0.05 level.
Figure 3. Effects of blue and purple light treatments on osmotic adjustment substances of Tetrastigma hemsleyanum
图 4 蓝光和紫光对三叶青黄酮合成酶活性的影响
不同小写字母表示在P<0.05水平差异显著。
Figure 4. Effects of blue and purple light treatments on activities of flavonoids synthases of Tetrastigma hemsleyanum
Different lowercase letters indicate significant differences at P<0.05 level. PAL: phenylalaninase activity; CHS: Charles ketone synthetase activity; CHI: Charles ketone isomerase activity.
-
[1] JI T, JI W W, WANG J, et al. A comprehensive review on traditional uses, chemical compositions, pharmacology properties and toxicology of Tetrastigma hemsleyanum[J]. Journal of Ethnopharmacology, 2021, 264: 113247 doi: 10.1016/j.jep.2020.113247 [2] 钟良瑞, 林霜, 魏克民. 三叶青黄酮抗肺癌作用研究[J]. 中国药理学通报, 2016, 32(4): 480−483 doi: 10.3969/j.issn.1001-1978.2016.04.008ZHONG L R, LIN S, WEI K M. Inhibitory effects of Radix Tetrastigma Hemsleyani Flavone on growth and invasion of lung carcinoma cells[J]. Chinese Pharmacological Bulletin, 2016, 32(4): 480−483 doi: 10.3969/j.issn.1001-1978.2016.04.008 [3] 廖淑彬, 蔡韦炜, 陈丹, 等. 闽产三叶青地上部分提取物体内抗炎镇痛作用研究[J]. 中国现代应用药学, 2017, 34(3): 319−324LIAO S B, CAI W W, CHEN D, et al. Anti-inflammatory and analgesic effects of the extracts of Tetrastigmatis hemsleyanum’s aerial parts from Fujianin vivo[J]. Chinese Journal of Modern Applied Pharmacy, 2017, 34(3): 319−324 [4] 李小明, 潘俊倩, 落艳娇, 等. 不同光强下光质对拟巫山淫羊藿生长发育和淫羊藿苷类黄酮含量的影响[J]. 中国中药杂志, 2020, 45(11): 2502−2508LI X M, PAN J Q, LUO Y J, et al. Effects of light quality on growth and icariin flavonoid content of Epimedium pseudowushanense under different light intensity[J]. China Journal of Chinese Materia Medica, 2020, 45(11): 2502−2508 [5] 林燕燕, 刘江洪, 戴秋香, 等. 提高茶树鲜叶儿茶素含量的不同光质萎凋工艺研究[J]. 热带作物学报, 2021, 42(12): 3605−3616 doi: 10.3969/j.issn.1000-2561.2021.12.030LIN Y Y, LIU J H, DAI Q X, et al. Withering technology of different light quality to increase catechin content in fresh tea leaves[J]. Chinese Journal of Tropical Crops, 2021, 42(12): 3605−3616 doi: 10.3969/j.issn.1000-2561.2021.12.030 [6] 胡瑜辉, 杨振宇, 宋诗娟, 等. 不同光质对黄芪抗氧化酶活性及产量和品质的影响[J]. 山西农业科学, 2021, 49(10): 1173−1178 doi: 10.3969/j.issn.1002-2481.2021.10.07HU Y H, YANG Z Y, SONG S J, et al. Effects of different light quality on antioxidant enzyme activity, yield and quality of Astragalus membranaceus var. mongholicus[J]. Journal of Shanxi Agricultural Sciences, 2021, 49(10): 1173−1178 doi: 10.3969/j.issn.1002-2481.2021.10.07 [7] 程小燕, 杨志玲, 杨旭, 等. 不同光质对三叶崖爬藤生长及有效成分含量的影响[J]. 林业科学研究, 2018, 31(5): 98−103CHENG X Y, YANG Z L, YANG X, et al. Effects of light quality on the growth and content of active ingredients of Tetrastigma hemsleyanum[J]. Forest Research, 2018, 31(5): 98−103 [8] 韩敏琪, 徐琳煜, 白岩, 等. 光质对三叶崖爬藤光合特性和总黄酮含量调控的研究[J]. 植物生理学报, 2019, 55(6): 883−890 doi: 10.13592/j.cnki.ppj.2018.0440HAN M Q, XU L Y, BAI Y, et al. Study on the regulation of light quality on photosynthetic characteristics and total flavonoid content of Tetrastigma hemsleyanum[J]. Plant Physiology Journal, 2019, 55(6): 883−890 doi: 10.13592/j.cnki.ppj.2018.0440 [9] BAI Y, CHEN W, LIU S Z, et al. Physiological responses of the Tetrastigma hemsleyanum plant under different color films[J]. HortScience, 2021, 56(6): 672−677 doi: 10.21273/HORTSCI15690-21 [10] BAI Y, GU Y W LIU S Z, et al. Flavonoids metabolism and physiological response to ultraviolet treatments in Tetrastigma hemsleyanum Diels et Gilg[J]. Frontiers in Plant Science, 2022, 13: 926197 doi: 10.3389/fpls.2022.926197 [11] LICHTENTHALER H K. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes[J]. Methods in Enzymology, 1987, 148: 350−382 [12] 张志良, 瞿伟菁, 李小方. 植物生理实验指导[M]. 北京: 高等教育出版社, 2009ZHANG Z L, QU W J, LI X F. Guidance of Plant Physiological Experiment[M]. Beijing: Higher Education Press, 2009 [13] 徐琳煜, 刘守赞, 白岩, 等. 不同光强处理对三叶青光合特性的影响[J]. 浙江农林大学学报, 2018, 35(3): 467−475 doi: 10.11833/j.issn.2095-0756.2018.03.010XU L Y, LIU S Z, BAI Y, et al. Effects of light intensity treatments on photosynthetic characteristics in Tetrastigma hemsleyanum[J]. Journal of Zhejiang A & F University, 2018, 35(3): 467−475 doi: 10.11833/j.issn.2095-0756.2018.03.010 [14] 胡晓甜, 刘守赞, 白岩, 等. 遮阴对浙江三叶青生理生化及总黄酮的影响[J]. 广西植物, 2019, 39(7): 925−932 doi: 10.11931/guihaia.gxzw201803056HU X T, LIU S Z, BAI Y, et al. Effects of different shading treatments on physiology, biochemistry and total flavonoids of Tetrastigma hemsleyanum in Zhejiang Province[J]. Guihaia, 2019, 39(7): 925−932 doi: 10.11931/guihaia.gxzw201803056 [15] 熊伟任, 雷雨田, 杨永森, 等. 不同波长蓝光对紫叶生菜品质形成的影响[J]. 江苏农业科学, 2023, 51(1): 143−148 doi: 10.15889/j.issn.1002-1302.2023.01.021XIONG W R, LEI Y T, YANG Y S, et al. Effect of blue light at different wavelengths on quality formation of Lactuca sativa[J]. Jiangsu Agricultural Sciences, 2023, 51(1): 143−148 doi: 10.15889/j.issn.1002-1302.2023.01.021 [16] 陈雷, 常丽, 曹福亮, 等. 银杏叶黄酮类化合物含量及相关酶活性对温度和干旱胁迫的响应[J]. 西北植物学报, 2013, 33(4): 755−762 doi: 10.3969/j.issn.1000-4025.2013.04.017CHEN L, CHANG L, CAO F L, et al. Effects of temperature and soil water deficit on the flavonoid content and activities of enzymes involved in Ginkgo leaves[J]. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(4): 755−762 doi: 10.3969/j.issn.1000-4025.2013.04.017 [17] 居鑫, 陈沁, 陈景斌, 等. 光质对小豆芽苗菜生长和类黄酮的影响及其机理初步探究[J]. 食品工业科技, 2019, 40(16): 64−70JU X, CHEN Q, CHEN J B, et al. Effects of light quality on growth and flavonoids of adzuki bean sprouts and its preliminary mechanism[J]. Science and Technology of Food Industry, 2019, 40(16): 64−70 [18] 招雪晴, 苑兆和. 石榴果色合成相关基因CHS和CHI的表达特性分析[J]. 基因组学与应用生物学, 2019, 38(5): 2175−2182ZHAO X Q, YUAN Z H. Expression profiles of fruit color related gene CHS and CHI in Punica granatum L.[J]. Genomics and Applied Biology, 2019, 38(5): 2175−2182 [19] LIU Y, FANG S, YANG W, et al. Light quality affects flavonoid production and related gene expression in Cyclocarya paliurus[J]. Journal of Photochemistry and Photobiology B: Biology, 2018, 179: 66−73 doi: 10.1016/j.jphotobiol.2018.01.002 [20] 范庆书, 庄晓蕾. 植物中紫外光及蓝光信号转导的研究进展[J]. 河北农业大学学报, 2001, 24(1): 95−98FAN Q S, ZHUANG X L. Progress on the UV and blue light signal transduction in plants[J]. Journal of Hebei Agricultural University, 2001, 24(1): 95−98 [21] 潘师峰, 徐志刚, 许亚良, 等. 光质对蔬菜作物类胡萝卜素合成与调控研究进展[J]. 中国蔬菜, 2023(1): 20−33PAN S F, XU Z G, XU Y L, et al. Research progress made in light quality on biosynthesis and regulation of carotenoids in vegetable crops[J]. China Vegetables, 2023(1): 20−33 [22] 汪星星, 陈钢, 左晓东, 等. 光质对杉木幼苗叶片光合作用的影响[J]. 安徽农业大学学报, 2022, 49(5): 716−723WANG X X, CHEN G, ZUO X D, et al. Optical reponse of photosynthesis of fir seedling under different light quality conditions[J]. Journal of Anhui Agricultural University, 2022, 49(5): 716−723 [23] 代艾林, 张永刚, 艾强, 等. 不同光质对朝鲜淫羊藿生长、生理和黄酮类成分积累的影响[J]. 中国农业科技导报, 2022, 24(4): 85−92DAI A L, ZHANG Y G, AI Q, et al. Effects of different light qualities on growth, physiological characteristics and flavonoids accumulation of Epimedium koreanum[J]. Journal of Agricultural Science and Technology, 2022, 24(4): 85−92 [24] 许珊珊, 唐银, 钟明慧, 等. 遮阴对粗肋草生长、光合特性和养分含量的影响[J]. 草业科学, 2022, 39(10): 2083−2094XU S S, TANG Y, ZHONG M H, et al. Effects of shading on the growth, photosynthetic characteristics and nutrient content of Aglaonema commutatum[J]. Pratacultural Science, 2022, 39(10): 2083−2094 [25] BAI Y, JIANG L T, LI Z, et al. Flavonoid metabolism in Tetrastigma hemsleyanum Diels et Gilg based on metabolome analysis and transcriptome sequencing[J]. Molecules, 2022, 28(1): 83 doi: 10.3390/molecules28010083 [26] 刘双双, 王忠华. 葡萄花色素苷生物合成调控研究进展[J]. 科技创新导报, 2015, 12(2): 89−92 doi: 10.3969/j.issn.1674-098X.2015.02.059LIU S S, WANG Z H. Research progress of the regulation of grapevine anthocyanin biosynthesis[J]. Science and Technology Innovation Herald, 2015, 12(2): 89−92 doi: 10.3969/j.issn.1674-098X.2015.02.059 [27] 陈家琛, 陈钢, 戴嘉豪, 等. 不同光质配比对杉木幼苗光合生理特征的影响[J]. 西南农业学报, 2022, 35(10): 2319−2325CHEN J S, CHEN G, DAI J H, et al. Effects of different light quality ratios on photosynthetic and physiological characteristics of Chinese fir seedlings[J]. Southwest China Journal of Agricultural Sciences, 2022, 35(10): 2319−2325 [28] 卢素萍, 赵铭钦, 刘鹏飞, 等. 不同光质条件下烤烟叶片衰老的生理效应研究[J]. 西南农业学报, 2016, 29(7): 1595−1599 doi: 10.16213/j.cnki.scjas.2016.07.017LU S P, ZHAO M Q, LIU P F, et al. Physiology effects of light quality on leaf senescence in flue-cured tobacco leaves[J]. Southwest China Journal of Agricultural Sciences, 2016, 29(7): 1595−1599 doi: 10.16213/j.cnki.scjas.2016.07.017 [29] 吴娇, 童玉珍, 周华, 等. 不同LED光配比对红绿线椒果实品质的影响[J]. 黑龙江农业科学, 2021, 9: 58−61WU J, TONG Y Z, ZHOU H, et al. Effects of LED light ratio on fruit nutrition quality of red and green line pepper[J]. Heilongjiang Agricultural Science, 2021, 9: 58−61 [30] 陈文, 刘守赞, 耿东杰, 等. 遮阴对浙北地区七叶一枝花生理生化指标及皂苷含量的影响[J]. 中国生态农业学报(中英文), 2022, 30(1): 72−81CHEN W, LIU S Z, GENG D J, et al. Effect of shading on saponin content and biochemical indexes of Paris polyphylla Smith var. chinensis (Franch.) Hara in northern Zhejiang[J]. Chinese Journal of Eco-Agriculture, 2022, 30(1): 72−81 [31] 张立伟, 刘世琦, 张自坤, 等. 不同光质下香椿苗的生长动态[J]. 西北农业学报, 2010, 19(6): 115−119 doi: 10.7606/j.issn.1004-1389.2010.06.-24ZHANG L W, LIU S Q, ZHANG Z K, et al. Dynamic of different qualities on growth of Toona sinensis seedlings[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(6): 115−119 doi: 10.7606/j.issn.1004-1389.2010.06.-24 [32] 吴飞洋, 柳新红, 董峰平, 等. 光照和土壤对乌桕秋季叶片色素及可溶性糖的影响[J]. 西南林业大学学报(自然科学), 2019, 39(6): 41−48WU F Y, LIU X H, DONG F P, et al. Effects of light and soil on pigment and soluble sugar in leaves of Sapium sebiferum[J]. Journal of Southwest Forestry University, 2019, 39(6): 41−48 [33] 刘鑫, 张亚红, 袁苗, 等. 红蓝光对设施‘红地球’葡萄花芽分化的影响[J]. 西南农业学报, 2023, 36(3): 637–646LIU X, ZHANG Y H, YUAN M, et al. Effect of red and blue light on flower bud differentiation of ‘Red Globe’ grape in protected culture[J]. Southwest China Journal of Agricultural Sciences, 2023, 36(3): 637–646 [34] 李慧敏, 陆晓民. 紫光光质对采后番茄果实品质的影响[J]. 安徽科技学院学报, 2017, 31(3): 20−24 doi: 10.19608/j.cnki.1673-8772.2017.0005LI H M, LU X M. Effects of purple light on fruit quality characteristic of post-harvest tomatoes[J]. Journal of Anhui Science and Technology University, 2017, 31(3): 20−24 doi: 10.19608/j.cnki.1673-8772.2017.0005 [35] 潘俊倩, 佟曦然, 郭宝林. 光对植物黄酮类化合物的影响研究进展[J]. 中国中药杂志, 2016, 21: 3897−3903PAN J Q, TONG X R, GUO B L. Progress of effects of light on plant flavonoids[J]. China Journal of Chinese Materia Medica, 2016, 21: 3897−3903 [36] 李青竹, 蔡友铭, 杨贞, 等. 不同LED光质对石蒜幼苗生长、生理和生物碱积累的影响[J]. 应用与环境生物学报, 2019, 25(6): 1414−1419 doi: 10.19675/j.cnki.1006-687x.2019.01018LI Q Z, CAI Y M, YANG Z, et al. Effects of the quality of LED light on the growth, physiological characteristics, and the accumulation of alkaloids in Lycoris radiata[J]. Chinese Journal of Applied and Environmental Biology, 2019, 25(6): 1414−1419 doi: 10.19675/j.cnki.1006-687x.2019.01018 [37] 孟畅, 赵杨, 杨菊, 等. 光质对皂荚幼苗生长发育、光合特性及相关生理指标的影响[J]. 植物生理学报, 2022, 58(10): 1961−1972 doi: 10.13592/j.cnki.ppj.2021.0383MENG C, ZHAO Y, YANG J, et al. Effects of light quality on growth and chlorophyll fluorescence parameters of Gleditsia sinensis seedlings[J]. Plant Physiology Journal, 2022, 58(10): 1961−1972 doi: 10.13592/j.cnki.ppj.2021.0383 -
下载:
点击查看大图
图(5)
计量
- 文章访问数: 262
- HTML全文浏览量: 143
- PDF下载量: 21
- 被引次数: 0
