Effect of increased plant density with reduced nitrogen on yield formation and nitrogen use efficiency of hybrid rice under high temperature and high humidity conditions
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摘要: 探明高温高湿稻区增密减氮对杂交稻产量形成和氮肥利用率的影响, 可为高温高湿稻区氮肥优化管理和合理密植提供依据。本研究以杂交稻‘内6优107’为材料, 于2018—2019年在典型的高温高湿稻区四川省泸州市进行大田试验。试验设6个密度与施氮量组合, 分别为低密高氮(习惯移栽密度16.5 万穴∙hm−2, 施氮量为180 kg∙hm−2, LDNck)、低密减氮15% (LDN−15%)、低密减氮30% (LDN−30%)、增密减氮15% (增密27%, HDN−15%)、增密减氮30% (HDN−30%)和低密不施氮(LDN0)。结果表明: 不同密肥组合对杂交稻产量影响显著(P<0.01)。与LDNck相比, HDN−15%和HDN−30%杂交稻产量分别增加4.3%~4.9%和2.3%~3.6%, 其优势主要表现在每穗粒数、结实率、花前干物质转运量、花前干物质转运效率、花前干物质转运对产量的贡献率和收获指数上。LDN−15%和LDN−30%杂交稻产量较LDNck分别降低2.3%~2.5%和4.8%~5.0%, 较低的有效穗、干物质、花后干物质积累及花后干物质积累对产量的贡献率是其减产的主要原因。HDN−15%和HDN−30%杂交稻花后氮素积累量、成熟期氮素吸收量低于LDNck处理, 但其花前氮素转运量、花前氮素转运效率、花前氮素转运贡献率、氮素干物质生产效率、氮素籽粒生产效率和氮素收获指数均高于LDNck处理, 因而HDN−15%和HDN−30%处理每生产100 kg稻谷需氮量分别减少6.8%~8.4%和9.0%~9.9%。与LDNck处理相比, HDN−15%和HDN−30%杂交稻氮肥农学利用率分别增加36.7%~37.4%和55.5%~60.4%、氮肥偏生产力增加22.8%~23.5%和46.3%~48.2%、氮肥吸收利用率增加5.6%~12.0%和17.0%~20.0%。可见, 在高温高湿稻区杂交稻生产上宜采用栽插密度为21.0万穴∙hm−2和施氮量为126~153 kg∙hm−2的组合。Abstract: The effects of increased plant density with reduced nitrogen (N) application rate on yield formation and nitrogen use efficiency (NUE) of hybrid rice were studied to provide a theoretical basis for optimum nitrogen fertilizer management and plant density under high temperature with high humidity conditions. Field experiments were conducted in Luzhou City from 2018 to 2019. The high yield and high quality hybrid rice variety ‘Nei6you107’ was grown under six combinations of plant density and N application rate: 1) locally recommended combination with a plant density of 16.5×104 hills∙hm−2 and a N rate of 180 kg∙hm−2 (LDNck); 2) combination of a plant density of 16.5×104 hills∙hm−2 and a reduced N rate by 15% (153 kg∙hm−2, LDN−15%); 3) combination of a plant density of 16.5×104 hills∙hm−2 and a reduced N rate by 30% (126 kg∙hm−2, LDN−30%); 4) combination of a increased plant density by about 27% (21.0×104 hills hm−2) and a reduced N rate by 15% (153 kg∙hm−2, HDN−15%); 5) combination of a increased plant density by about 27% (21.0×104 hills∙hm−2) and a reduced N rate by 30% (126 kg∙hm−2 HDN−30%); and 6) combination of a plant density of 16.5×104 hills∙hm−2 and zero N rate (LDN0). The grain yield, yield components, dry matter, N uptake and NUE were measured. The results showed that the grain yield of hybrid rice was significantly affected by different combinations of plant density and N rate (P<0.01). HDN−15% and HDN−30% produced higher grain yields than LDNck by 4.3%−4.9% and 2.3%−3.6%, respectively. The higher grain yields under HDN−15% and HDN−30% were attributed to improvement in spikelets per panicle, grain filling rate, translocation of dry matter accumulated at heading stage (TDMHD), translocation percentage of dry matter accumulated at heading stage (TPDMHD), contribution percentage of pre-anthesis dry matter translocation to grain yield (CPDMTGHD) and harvest index. The LDN−15% and LDN−30% had 2.3%−2.5% and 4.8%−5.0% lower grain yield than LDNck, respectively. The yield gap between LDN−15%, LDN−30% and LDNck was attributed to the difference in effective panicles, total dry matter, dry matter accumulation from heading to maturity, and contribution percentage of dry matter accumulation from heading to maturity stage to grain yield (CPDMGHD-MA). The HDN−15% and HDN−30% had lower nitrogen accumulation from heading to maturity and total N uptake than LDNck, whereas the translocation of N accumulated at heading stage (NTGNHD), translocation percentage of N accumulated at heading stage (TPNHD), contribution percentage of pre-anthesis N accumulation translocation to grain N accumulation (CPNTGNHD), N use efficiency for biomass production (NUEBP), N use efficiency for grain production (NUEGP) and N harvest index under HDN−15% and HDN−30% were higher than those under LDNck. Consequently, HDN−15% and HDN−30% had lower N requirements to produce 100 kg of grain (NRPG) than LDNck by 6.8%−8.4% and 9.0%−9.9%, respectively. HDN−15% enhanced the agronomic efficiency of applied N (AEN) by 36.7%−37.4%, partial factor productivity of applied N (PFPN) by 22.8%−23.5% and recovery efficiency of applied N (REN) by 5.6%−12.0% over LDNck. The HDN−30% produced higher AEN, PFPN and REN than LDNck by 55.5%−60.4%, 46.3%−48.2% and 17.0%−20.0%, respectively. The rational combination of plant density and N rate can improve panicle number per unit area, grain filling, TDMHD, TPDMHD, NTGNHD, TPNHD and harvest index, which further increasing the grain yield and NUE. The optimum combination is plant density of 21.0×104 hills∙hm−2 plus N rate of 126−153 kg∙hm−2 in high temperature with high humidity condition.
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表 1 高温高湿区不同密肥处理对杂交稻产量及其构成的影响
Table 1. Effect of combination of plant density and N rate on grain yield and yield components of hybrid rice under high temperature and high humidity conditions
年份
Year处理 Treatment 有效穗数
Effective panicles
(panicles∙m−2)每穗粒数
Grains number
per panicle结实率
Grain filling
(%)粒重
Grain weight
(mg)产量
Grain yield
(t∙hm−2)代码
Code密度
Plant density
(×104 holes∙hm−2)施氮量
N application rate
(kg∙hm−2)2018 LDN0 16.5 0 155.3±13.7c 138.0±4.2c 89.5±1.9a 28.1±0.2b 6.22±0.42d LDN−15% 16.5 153 181.4±9.8b 158.8±4.8a 86.5±1.7a 29.7±0.5a 8.31±0.21bc LDN−30% 16.5 126 179.2±10.4bc 154.0±3.9ab 88.4±3.0a 29.3±0.3a 8.11±0.14c HDN−15% 21.0 153 199.7±9.1ab 148.5±2.4b 90.1±1.0a 29.3±0.5a 8.89±0.11a HDN−30% 21.0 126 179.3±4.4bc 160.4±5.1a 90.6±0.9a 29.3±0.6a 8.72±0.37ab LDNCK 16.5 180 206.4±22.6a 145.5±6.5bc 86.1±5.9a 29.4±0.9a 8.52±0.70abc 2019 LDN0 16.5 0 179.2±12.9c 159.3±7.1a 80.9±2.4b 28.1±0.3ab 6.33±0.05c LDN−15% 16.5 153 205.4±5.2b 149.3±10.9ab 84.5±1.6ab 27.7±0.6b 8.62±0.25b LDN−30% 16.5 126 204.1±21.2bc 147.2±11.2ab 85.4±2.0a 28.8±0.2a 8.38±0.15b HDN−15% 21.0 153 237.6±9.1a 138.2±1.8b 84.3±3.1ab 28.5±0.5ab 9.25±0.13a HDN−30% 21.0 126 234.7±16.6a 141.1±3.9b 84.9±1.4ab 28.4±0.9ab 9.14±0.23a LDNCK 16.5 180 230.2±25.1ab 137.5±2.6b 84.0±3.0ab 27.9±0.5ab 8.82±0.50ab 方差分析 Analysis of variance 年份 Year (Y) ** ** * ** ** 处理 Treatment (T) ** ** ns ns ** 年份×处理 Y×T ns ** ns ns ns 同年同列数据后不同字母表示处理间在P<0.05水平差异显著。*表示差异达P<0.05显著水平, **表示差异达P<0.01显著水平, ns 表示差异不显著。Values followed by different letters in a year within a column are significantly different at P<0.05 level. * and ** mean significance at P<0.05 and P<0.01 levels, respectively. ns denotes non-significance. 表 2 高温高湿区不同密肥处理对杂交稻干物质生产及收获指数的影响
Table 2. Effect of combination of plant density and N rate on biomass production of hybrid rice under high temperature and high humidity condition
年份
Year处理 Treatment 干物质 Dry matter (g∙m−2) 总干物质
Total dry matter (g∙m−2)收获指数
Harvest index (%)代码
Code密度
Plant density
(×104 holes∙hm−2)施氮量
N application rate
(kg∙hm−2)开花前
Before heading开花后
After heading2018 LDN0 16.5 0 612.3±30.2c 280.6±44.4b 892.8±60.9c 60.3±0.9a LDN−15% 16.5 153 981.7±13.1ab 379.2±24.9a 1360.9±37.3a 54.3±0.8b LDN−30% 16.5 126 931.8±35.0b 304.1±53.7ab 1235.9±72.7b 57.8±1.3ab HDN−15% 21.0 153 1013.1±44.7a 307.4±28.9ab 1320.5±56.6ab 59.3±1.5a HDN−30% 21.0 126 968.2±72.5ab 359.5±105.4ab 1327.8±40.6ab 57.5±2.5ab LDNCK 16.5 180 978.5±8.7ab 381.6±29.0a 1360.1±37.2a 55.5±3.6b 2019 LDN0 16.5 0 846.3±43.8b 299.6±77.9ab 1145.9±38.9d 56.6±0.4a LDN−15% 16.5 153 1130.1±10.9a 278.4±34.5ab 1408.5±44.0ab 52.5±2.2b LDN−30% 16.5 126 1106.5±26.9a 207.4±74.2b 1313.9±50.7c 56.0±1.8a HDN−15% 21.0 153 1137.9±32.0a 337.8±35.6a 1475.7±19.6a 53.3±0.7b HDN−30% 21.0 126 1102.8±23.6a 285.7±44.4ab 1388.5±21.2b 55.9±0.4a LDNCK 16.5 180 1136.3±14.6a 316.9±46.9ab 1453.2±34.5ab 51.7±0.8b 方差分析 Analysis of variance 年份 Year (Y) ** * ** ** 处理 Treatment (T) ** * ** ** 年份×处理 Y×T ns ns ** ns 同年同列数据后不同字母表示处理间在P<0.05水平差异显著。*表示差异达P<0.05显著水平, **表示差异达P<0.01显著水平, ns 表示差异不显著。Values followed by different letters in a year within a column are significantly different at P<0.05 level. * and ** mean significance at P<0.05 and P<0.01 levels, respectively. ns denotes non-significance. 表 3 高温高湿区不同密肥处理对杂交稻花前干物质转运的影响
Table 3. Effect of combination of plant density and N rate on translocation of dry matter of hybrid rice at heading stage under high temperature and high humidity condition
年份
Year处理 Treatment 花前干物质
转运量
TDMHD (g∙m−2)花前干物质转
运效率
TPDMHD (g∙m−2)花前干物质转运
对产量的贡献率
CPDMTGHD (g∙m−2)花后干物质积累
对产量的贡献率
CPDMGHD-MA (g∙m−2)代码
Code密度
Plant density
(×104 holes∙hm−2)施氮量
N application rate
(kg∙hm−2)2018 LDN0 16.5 0 341.4±73.2c 55.7±11.7ab 54.6±8.8a 45.4±8.8a LDN−15% 16.5 153 452.1±15.0b 46.1±2.1b 54.4±2.2a 45.6±2.2a LDN−30% 16.5 126 507.3±67.2ab 54.5±8.0ab 62.4±7.3a 37.6±7.3a HDN−15% 21.0 153 581.7±30.9a 57.4±3.4a 65.4±3.3a 34.6±3.3a HDN−30% 21.0 126 512.7±75.7ab 52.7±3.8ab 59.0±10.5a 41.0±10.5a LDNCK 16.5 180 470.6±9.7b 48.1±1.1ab 55.3±2.2a 44.7±2.2a 2019 LDN0 16.5 0 333.3±74.4b 39.2±6.8b 52.7±12.0b 47.3±12.0a LDN−15% 16.5 153 583.2±30.8a 51.6±3.2a 67.7±3.6a 32.3±3.6b LDN−30% 16.5 126 630.6±89.3a 56.9±6.8a 75.1±9.3a 24.9±9.3b HDN−15% 21.0 153 587.5±25.8a 51.6±1.7a 63.5±3.4ab 36.5±3.4ab HDN−30% 21.0 126 628.6±65.4a 56.9±4.8a 68.7±5.6a 31.3±5.6b LDNCK 16.5 180 565.3±91.9a 49.7±4.8a 63.9±5.6ab 36.1±5.6ab 方差分析 Analysis of variance 年份 Year (Y) ** ns ** ** 处理 Treatment (T) ** ns * * 年份×处理 Y´T ns * ns ns 同年同列数据后不同字母表示处理间在P<0.05水平差异显著。*表示差异达P<0.05显著水平, **表示差异达P<0.01显著水平, ns 表示差异不显著。Values followed by different letters in a year within a column are significantly different at P<0.05 level. * and ** mean significance at P<0.05 and P<0.01 levels, respectively. ns denotes non-significance. TDMHD, TPDMHD, CPDMTGHD, CPDMGHD-MA represent translocation of dry matter accumulated at heading stage, translocation percentage of dry matter accumulated at heading stage, contribution percentage of pre-anthesis dry matter translocation to grain yield, contribution percentage of dry matter accumulation from heading to maturity stage to grain yield, respectively. 表 4 高温高湿区不同密肥处理对杂交稻植株氮素吸收积累的影响
Table 4. Effect of combination of plant density and N rate on N uptake of hybrid rice under high temperature and high humidity condition
年份
Year处理 Treatment 氮素吸收量 N uptake (g∙m−2) 氮素总吸收量
Total N uptake
(g∙m−2)氮素收获指数
N harvest index (%)代码
Code密度
Plant density
(×104 holes∙hm−2)施氮量
N application rate
(kg∙hm−2)开花前
Before heading开花后
After heading2018 LDN0 16.5 0 6.2±0.5d 2.5±1.0abc 8.7±0.5d 75.3±4.4a LDN−15% 16.5 153 12.6±0.2a 1.4±0.4c 14.0±0.2bc 68.6±0.7cd LDN−30% 16.5 126 9.8±0.8c 3.4±0.9ab 13.2±0.8c 70.0±1.7bcd HDN−15% 21.0 153 12.4±0.4a 2.1±0.6bc 14.5±0.9ab 71.9±2.2abc HDN−30% 21.0 126 11.5±0.5b 2.6±1.6abc 14.1±1.4bc 72.6±1.0ab LDNCK 16.5 180 11.6±0.5b 3.5±0.5a 15.1±0.9a 67.6±2.3d 2019 LDN0 16.5 0 6.4±0.4d 2.8±0.7a 9.1±0.4c 73.3±1.5a LDN−15% 16.5 153 11.4±0.2a 2.4±0.6a 13.8±0.5a 69.0±1.0b LDN−30% 16.5 126 10.6±0.7bc 2.3±1.2a 13.0±0.7b 69.7±1.4b HDN−15% 21.0 153 11.1±0.4ab 2.9±0.2a 14.0±1.0a 68.5±1.9b HDN−30% 21.0 126 10.2±0.4c 3.1±0.3a 13.3±0.4ab 71.2±2.1b LDNCK 16.5 180 10.9±0.2abc 3.4±0.5a 14.3±0.4ab 64.9±0.9c 方差分析 Analysis of variance 年份 Year (Y) ** ns ns * 处理 Treatment (T) ** * * ** 年份×处理 Y×T ** ns ns ns 同年同列数据后不同字母表示处理间在P<0.05水平差异显著。*表示差异达P<0.05显著水平, **表示差异达P<0.01显著水平, ns 表示差异不显著。Values followed by different letters in a year within a column are significantly different at P<0.05 level. * and ** mean significance at P<0.05 and P<0.01 levels, respectively. ns denotes non-significance. 表 5 高温高湿区不同密肥处理对杂交稻齐穗期茎叶氮素运转的影响
Table 5. Effect of combination of plant density and N rate on nitrogen translocation of stem and leaf of hybrid rice under high temperature and high humidity condition
年份
Year处理 Treatment 氮素转运量
N translocation
(g∙m−2)氮素表观转运率
Apparent N translocation
rate (%)氮素转运贡献率
N translocate contribution
rate (%)代码
Code密度
Plant density
(×104 holes∙hm−2)施氮量
N application rate
(kg∙hm−2)2018 LDN0 16.5 0 2.8±0.8c 56.2±10.5abc 43.1±12.2d LDN−15% 16.5 153 6.6±0.2a 60.0±1.4ab 68.8±2.4a LDN−30% 16.5 126 4.3±0.8b 51.9±3.4bc 46.7±9.6cd HDN−15% 21.0 153 6.4±0.8a 61.4±2.3a 62.0±1.3ab HDN−30% 21.0 126 5.8±0.3a 60.1±1.4ab 57.0±1.6bc LDNCK 16.5 180 4.8±0.6b 49.2±4.9c 46.5±4.6cd 2019 LDN0 16.5 0 2.8±0.5c 52.8±4.0a 41.5±9.7b LDN−15% 16.5 153 4.9±0.3ab 53.1±2.7a 51.0±3.2ab LDN−30% 16.5 126 5.0±0.6a 55.9±2.9a 55.7±9.9a HDN−15% 21.0 153 4.8±0.5ab 52.3±3.8a 50.4±3.2ab HDN−30% 21.0 126 4.6±0.5ab 54.6±1.4ab 48.2±2.6ab LDNCK 16.5 180 4.0±0.4b 44.6±3.2b 43.7±5.1ab 方差分析 Analysis of variance 年份 Year (Y) ** ** * 处理 Treatment (T) ** ** ** 年份×处理 Y×T ** ns * 同年同列数据后不同字母表示处理间在P<0.05水平差异显著。*表示差异达P<0.05显著水平, **表示差异达P<0.01显著水平, ns 表示差异不显著。Values followed by different letters in a year within a column are significantly different at P<0.05 level. * and ** mean significance at P<0.05 and P<0.01 levels, respectively. ns denotes non-significance. 表 6 高温高湿区不同密肥处理对杂交稻氮肥利用率的影响
Table 6. Effect of combination of plant density and N rate on nitrogen use efficiency of hybrid rice under high temperature and high humidity condition
年份
Year处理 Treatment 氮肥农学利用率
Agronomic efficiency of
applied N (kg∙kg−1)氮肥偏生产力
Partial factor productivity of
applied N (kg∙kg−1)氮肥吸收利用率
Recovery efficiency of
applied N (%)代码
Code密度
Plant density
(×104 holes∙hm−2)施氮量
N application rate
(kg∙hm−2)2018 LDN−15% 16.5 153 13.7±2.1c 54.3±1.4d 34.5±3.7b LDN−30% 16.5 126 15.0±3.6bc 64.4±1.1b 36.0±3.2ab HDN−15% 21.0 153 17.5±2.1ab 58.1±0.7c 38.2±4.5ab HDN−30% 21.0 126 19.9±3.2a 69.2±2.9a 43.4±9.5a LDNCK 16.5 180 12.8±3.1c 47.3±1.5e 36.2±1.8ab 2019 LDN−15% 16.5 153 14.9±2.0c 56.3±1.6d 30.7±3.1a LDN−30% 16.5 126 16.3±1.2bc 66.5±1.2b 30.5±7.8a HDN−15% 21.0 153 19.1±0.9b 60.5±0.8c 31.7±3.4a HDN−30% 21.0 126 22.3±1.9a 72.6±1.8a 33.1±5.5a LDNCK 16.5 180 13.9±3.0c 49.0±2.8e 28.3±4.1b 方差分析 Analysis of variance 年份 Year (Y) ns ** ** 处理 Treatment (T) * ** ns 年份×处理 Y×T ns ns ns 同年同列数据后不同字母表示处理间在P<0.05水平差异显著。*表示差异达P<0.05显著水平, **表示差异达P<0.01显著水平, ns 表示差异不显著。Values followed by different letters in a year within a column are significantly different at P<0.05 level. * and ** mean significance at P<0.05 and P<0.01 levels, respectively. ns denotes non-significance. 表 7 高温高湿区不同密肥处理对杂交稻每生产100 kg稻谷需氮量、氮素干物质生产效率、氮素籽粒生产效率的影响
Table 7. Effect of combination of plant density and N rate on N use efficiency for biomass and grain yield production and N requirement for produced 100 kg grain of hybrid rice under high temperature and high humidity condition
年份
Year处理 Treatment 100 kg稻谷需氮量
N requirement of 100 kg grains
(kg)氮素干物质生产效率
N use efficiency for biomass
production (kg∙kg−1)氮素籽粒生产效率
N use efficiency for grain
production (kg∙kg−1)代码
Code密度
Plant density
(×104 holes∙hm−2)施氮量
N application rate
(kg∙hm−2)2018 LDN0 16.5 0 1.39±0.19 b 103.8±9.0 a 72.5±9.1 a LDN−15% 16.5 153 1.67±0.04 a 97.8±1.1 ab 59.8±1.3 b LDN−30% 16.5 126 1.62±0.03 a 93.9±6.3 b 61.6±1.1 b HDN−15% 21.0 153 1.63±0.05 a 91.3±2.7 b 61.5±1.8 b HDN−30% 21.0 126 1.62±0.22 a 95.1±12.7 ab 62.5±9.2 b LDNCK 16.5 180 1.78±0.04 a 89.9±1.5 b 56.3±1.4 b 2019 LDN0 16.5 0 1.45±0.06 b 125.4±2.1 a 69.3±2.9 a LDN−15% 16.5 153 1.61±0.02 a 101.8±3.4 b 62.2±0.6 c LDN−30% 16.5 126 1.55±0.11 ab 101.3±5.5 b 64.7±4.5 bc HDN−15% 21.0 153 1.51±0.02 abc 105.4±0.8 b 66.1±1.0 abc HDN−30% 21.0 126 1.46±0.04 bc 104.4±4.2 b 68.7±1.7 ab LDNCK 16.5 180 1.62±0.04 a 102.2±5.3 b 61.9±1.7 c 方差分析 Analysis of variance 年份 Year (Y) ** * ** 处理 Treatment (T) ** ** ** 年份×处理 Y×T ns ns ns 同年同列数据后不同字母表示处理间在P<0.05水平差异显著。*表示差异达P<0.05显著水平, **表示差异达P<0.01显著水平, ns 表示差异不显著。Values followed by different letters in a year within a column are significantly different at P<0.05 level. * and ** mean significance at P<0.05 and P<0.01 levels, respectively. ns denotes non-significance -
[1] 邹应斌, 周上游, 唐起源. 中国超级杂交水稻超高产栽培研究的现状与展望[J]. 中国农业科技导报, 2003, 5(1): 31−35 doi: 10.3969/j.issn.1008-0864.2003.01.007ZOU Y B, ZHOU S Y, TANG Q Y. Status and outlook of high yielding cultivation researches on China super hybrid rice[J]. Review of China Agricultural Science and Technology, 2003, 5(1): 31−35 doi: 10.3969/j.issn.1008-0864.2003.01.007 [2] 朱德峰, 林贤青, 曹卫星. 超高产水稻品种的根系分布特点[J]. 南京农业大学学报, 2000, 23(4): 5−8ZHU D F, LIN X Q, CAO W X. Characteristics of root distribution of super high yielding rice varieties[J]. Journal of Nanjing Agricultural University, 2000, 23(4): 5−8 [3] 袁小乐, 潘晓华, 石庆华, 等. 超级早、晚稻的养分吸收和根系分布特性研究[J]. 植物营养与肥料学报, 2010, 16(1): 27−32 doi: 10.11674/zwyf.2010.0105YUAN X Y, PAN X H, SHI Q H, et al. Characteristics of nutrient uptake and root system distribution in super early and super late rice[J]. Plant Nutrition and Fertilizer Science, 2010, 16(1): 27−32 doi: 10.11674/zwyf.2010.0105 [4] 朱铁忠, 柯健, 姚波, 等. 沿江双季北缘区机插早稻的超高产群体特征[J]. 中国农业科学, 2021, 54(7): 1553−1564 doi: 10.3864/j.issn.0578-1752.2021.07.018ZHU T Z, KE J, YAO B, et al. Super-high yield characteristics of mechanically transplanting double-cropping early rice in the northern margin area of Yangtze River[J]. Scientia Agricultura Sinica, 2021, 54(7): 1553−1564 doi: 10.3864/j.issn.0578-1752.2021.07.018 [5] JIANG P, XIE X B, HUANG M, et al. Potential yield increase of hybrid rice at five locations in Southern China[J]. Rice, 2016, 9(1): 1−14 doi: 10.1186/s12284-015-0073-2 [6] 邹应斌. 长江流域双季稻栽培技术发展[J]. 中国农业科学, 2011, 44(2): 254−262 doi: 10.3864/j.issn.0578-1752.2011.02.004ZOU Y B. Development of cultivation technology for double cropping rice along the Changjiang River valley[J]. Scientia Agricultura Sinica, 2011, 44(2): 254−262 doi: 10.3864/j.issn.0578-1752.2011.02.004 [7] PENG S B, TANG Q Y, ZOU Y B. Current status and challenges of rice production in China[J]. Plant Production Science, 2009, 12(1): 3−8 doi: 10.1626/pps.12.3 [8] 朱兆良. 我国土壤供氮和化肥氮去向研究的进展[J]. 土壤, 1985, 17(1): 2−9ZHU Z L. Research in soil supply nitrogen and fate of fertilizer nitrogen Chinese[J]. Soils, 1985, 17(1): 2−9 [9] WANG G H, DOBERMANN A, WITT C, et al. Performance of site-specific nutrient management for irrigated rice in southeast China[J]. Agronomy Journal, 2001, 93(4): 869−878 doi: 10.2134/agronj2001.934869x [10] HUANG M, YANG C L, JI Q M, et al. Tillering responses of rice to plant density and nitrogen rate in a subtropical environment of Southern China[J]. Field Crops Research, 2013, 149: 187−192 doi: 10.1016/j.fcr.2013.04.029 [11] AO H J, XIE X B, HUANG M, et al. Decreasing hill density combined with increasing nitrogen rate led to yield decline in hybrid rice under low-light conditions[J]. Scientific Reports, 2019, 9: 15786 doi: 10.1038/s41598-019-52376-2 [12] 朱兆良, 金继运. 保障我国粮食安全的肥料问题[J]. 植物营养与肥料学报, 2013, 19(2): 259−273 doi: 10.11674/zwyf.2013.0201ZHU Z L, JIN J Y. Fertilizer use and food security in China[J]. Plant Nutrition and Fertilizer Science, 2013, 19(2): 259−273 doi: 10.11674/zwyf.2013.0201 [13] PENG S B, BURESH R J, HUANG J L, et al. Improving nitrogen fertilization in rice by sitespecific N management. A review[J]. Agronomy for Sustainable Development, 2010, 30(3): 649−656 doi: 10.1051/agro/2010002 [14] WU W, NIE L X, LIAO Y C, et al. Toward yield improvement of early-season rice: Other options under double rice-cropping system in central China[J]. European Journal of Agronomy, 2013, 45: 75−86 doi: 10.1016/j.eja.2012.10.009 [15] 谢小兵, 王玉梅, 黄敏, 等. 单本密植机插对杂交稻生长和产量的影响[J]. 作物学报, 2016, 42(6): 924−931 doi: 10.3724/SP.J.1006.2016.00924XIE X B, WANG Y M, HUANG M, et al. Effect of mechanized transplanting with high hill density and single seedling per hill on growth and grain yield in hybrid rice[J]. Acta Agronomica Sinica, 2016, 42(6): 924−931 doi: 10.3724/SP.J.1006.2016.00924 [16] HUANG M, CHEN J N, CAO F B, et al. Increased hill density can compensate for yield loss from reduced nitrogen input in machine-transplanted double-cropped rice[J]. Field Crops Research, 2018, 221: 333−338 doi: 10.1016/j.fcr.2017.06.028 [17] 马金玉, 梁宏, 罗勇, 等. 中国近50年太阳直接辐射和散射辐射变化趋势特征[J]. 物理学报, 2011, 60(6): 069601 doi: 10.7498/aps.60.069601MA J Y, LIANG H, LUO Y, et al. Variation trend of direct and diffuse radiation in China over recent 50 years[J]. Acta Physica Sinica, 2011, 60(6): 069601 doi: 10.7498/aps.60.069601 [18] 周开达. 四川水稻超高产育种的发展趋势[J]. 西南农业学报, 1998, 11(S2): 1ZHOU K D. Trends of super high yield breeding in rice (Oryza sativa L.) in Sichuan Province[J]. Southwest China Journal of Agricultural Sciences, 1998, 11(S2): 1 [19] 周开达, 马玉清, 刘太清, 等. 杂交水稻亚种间重穗型组合选育−杂交水稻超高产育种的理论与实践[J]. 四川农业大学学报, 1995, 13(4): 403−407ZHOU K D, MA Y Q, LIU T Q, et al. The breeding of subspecific heavy ear hybrid rice—exploration about super-high yield breeding of hybrid rice[J]. Journal of Sichuan Agricultural University, 1995, 13(4): 403−407 [20] 徐富贤, 熊洪, 朱永川, 等. 川东南冬水田杂交中稻进一步高产的栽培策略[J]. 作物学报, 2007, 33(6): 1004−1009 doi: 10.3321/j.issn:0496-3490.2007.06.023XU F X, XIONG H, ZHU Y C, et al. Cultivation strategy of hybrid mid-season rice for further high yield in winter water-logged field in the southeast of Sichuan Province[J]. Acta Agronomica Sinica, 2007, 33(6): 1004−1009 doi: 10.3321/j.issn:0496-3490.2007.06.023 [21] 陈佳娜, 曹放波, 谢小兵, 等. 机插条件下低氮密植栽培对“早晚兼用”双季稻产量和氮素吸收利用的影响[J]. 作物学报, 2016, 42(8): 1176−1187 doi: 10.3724/SP.J.1006.2016.01176CHEN J N, CAO F B, XIE X B, et al. Effect of low nitrogen rate combined with high plant density on yield and nitrogen use efficiency of machine-transplanted early-late season double cropping rice[J]. Acta Agronomica Sinica, 2016, 42(8): 1176−1187 doi: 10.3724/SP.J.1006.2016.01176 [22] 周建霞, 张玉屏, 朱德峰, 等. 空气湿度和土壤水分对高温诱导水稻颖花不育的影响[J]. 江西农业学报, 2017, 29(2): 24−27ZHOU J X, ZHANG Y P, ZHU D F, et al. Effects of air humidity and soil moisture on rice spikelet sterility induced by high temperature[J]. Acta Agriculturae Jiangxi, 2017, 29(2): 24−27 [23] 蒋鹏, 熊洪, 张林, 等. 不同生态条件下施氮量和移栽密度对杂交稻旌优127产量及稻米品质的影响[J]. 核农学报, 2017, 31(10): 2007−2015 doi: 10.11869/j.issn.100-8551.2017.10.2007JIANG P, XIONG H, ZHANG L, et al. Effects of nitrogen fertilization and planting density on grain yield and quality of Jingyou127 and rice quality under different ecological conditions[J]. Journal of Nuclear Agricultural Sciences, 2017, 31(10): 2007−2015 doi: 10.11869/j.issn.100-8551.2017.10.2007 [24] 马均, 朱庆森, 马文波, 等. 重穗型水稻光合作用、物质积累与运转的研究[J]. 中国农业科学, 2003, 36(4): 375−381 doi: 10.3321/j.issn:0578-1752.2003.04.004MA J, ZHU Q S, MA W B, et al. Studies on the photosynthetic characteristics and accumulation and transformation of assimilation product in heavy panicle type of rice[J]. Scientia Agricultura Sinica, 2003, 36(4): 375−381 doi: 10.3321/j.issn:0578-1752.2003.04.004 [25] YANG J C, PENG S B, ZHANG Z J, et al. Grain and dry matter yields and partitioning of assimilates in Japonica/indica hybrid rice[J]. Crop Science, 2002, 42(3): 766 doi: 10.2135/cropsci2002.0766 [26] 谢小兵, 蒋鹏, 黄敏, 等. 基于黄金分割法的双季稻合理密植研究[J]. 核农学报, 2016, 30(12): 2467−2476 doi: 10.11869/j.issn.100-8551.2016.12.2467XIE X B, JIANG P, HUANG M, et al. Study on rational close planting based on golden section method for double cropping rice[J]. Journal of Nuclear Agricultural Sciences, 2016, 30(12): 2467−2476 doi: 10.11869/j.issn.100-8551.2016.12.2467 [27] 徐新朋, 周卫, 梁国庆, 等. 氮肥用量和密度对双季稻产量及氮肥利用率的影响[J]. 植物营养与肥料学报, 2015, 21(3): 763−772 doi: 10.11674/zwyf.2015.0324XU X P, ZHOU W, LIANG G Q, et al. Effects of nitrogen and density interactions on grain yield and nitrogen use efficiency of double-rice systems[J]. Journal of Plant Nutrition and Fertilizer, 2015, 21(3): 763−772 doi: 10.11674/zwyf.2015.0324 [28] 黄巧义, 唐拴虎, 张发宝, 等. 减氮配施控释尿素对水稻产量和氮肥利用的影响[J]. 中国生态农业学报, 2017, 25(6): 829−838HUANG Q Y, TANG S H, ZHANG F B, et al. Effect of combined application of controlled-release urea and conventional urea under reduced N rate on yield and N utilization efficiency of rice[J]. Chinese Journal of Eco-Agriculture, 2017, 25(6): 829−838 [29] 肖小平, 李超, 唐海明, 等. 秸秆还田下减氮增密对双季稻田土壤氮素库容及氮素利用率的影响[J]. 中国生态农业学报(中英文), 2019, 27(3): 422−430XIAO X P, LI C, TANG H M, et al. Soil nitrogen storage and recovery efficiency in double paddy fields under reduced nitrogen dose and increased crop density[J]. Chinese Journal of Eco-Agriculture, 2019, 27(3): 422−430 [30] CHEN Y T, PENG J, WANG J, et al. Crop management based on multi-split topdressing enhances grain yield and nitrogen use efficiency in irrigated rice in China[J]. Field Crops Research, 2015, 184: 50−57 doi: 10.1016/j.fcr.2015.09.006 [31] PENG S B, BURESH R J, HUANG J L, et al. Strategies for overcoming low agronomic nitrogen use efficiency in irrigated rice systems in China[J]. Field Crops Research, 2006, 96(1): 37−47 doi: 10.1016/j.fcr.2005.05.004 [32] JIANG L G, DAI T B, JIANG D, et al. Characterizing physiological N-use efficiency as influenced by nitrogen management in three rice cultivars[J]. Field Crops Research, 2004, 88(2/3): 239−250 [33] QIAO J, YANG L Z, YAN T M, et al. Nitrogen fertilizer reduction in rice production for two consecutive years in the Taihu Lake area[J]. Agriculture, Ecosystems & Environment, 2012, 146(1): 103−112 [34] 敖和军, 王淑红, 邹应斌, 等. 不同施肥水平下超级杂交稻对氮、磷、钾的吸收累积[J]. 中国农业科学, 2008, 41(10): 3123−3132 doi: 10.3864/j.issn.0578-1752.2008.10.028AO H J, WANG S H, ZOU Y B, et al. Characteristics of nutrient uptake and utilization of super hybrid rice under different fertilizer application rates[J]. Scientia Agricultura Sinica, 2008, 41(10): 3123−3132 doi: 10.3864/j.issn.0578-1752.2008.10.028 [35] 徐富贤, 熊洪, 张林, 等. 西南稻区不同地域和施氮水平对杂交中稻氮、磷、钾吸收累积的影响[J]. 作物学报, 2011, 37(5): 882−894 doi: 10.3724/SP.J.1006.2011.00882XU F X, XIONG H, ZHANG L, et al. Characteristics of nutrient uptake and utilization of mid-season hybrid rice under different nitrogen application rates in different locations of southwest China[J]. Acta Agronomica Sinica, 2011, 37(5): 882−894 doi: 10.3724/SP.J.1006.2011.00882 [36] 蒋鹏, 熊洪, 张林, 等. 不同生态条件下施氮量和移栽密度对杂交稻氮、磷、钾吸收积累的影响[J]. 植物营养与肥料学报, 2017, 23(2): 342−350 doi: 10.11674/zwyf.16280JIANG P, XIONG H, ZHANG L, et al. Effects of N rate and planting density on nutrient uptake and utilization of hybrid rice under different ecological conditions[J]. Journal of Plant Nutrition and Fertilizer, 2017, 23(2): 342−350 doi: 10.11674/zwyf.16280