Meta analysis of the effects of foliar Se application on grain yield, protein content, and Se accumulation of winter wheat

NIE Haoliang; HUANG Shaohui; YANG Junfang; YANG Yunma; ZHANG Jing; YANG Huimin; YANG Wenfang; XING Suli; JIA Liangliang; YUE Zengliang

doi:10.12357/cjea.20230229

Volume 31 Issue 12

Dec. 2023

Turn off MathJax

Article Contents

Article Navigation > Chinese Journal of Eco-Agriculture > 2023 > 31(12): 1997-2010

NIE H L, HUANG S H, YANG J F, YANG Y M, ZHANG J, YANG H M, YANG W F, XING S L, JIA L L, YUE Z L. Meta analysis of the effects of foliar Se application on grain yield, protein content, and Se accumulation of winter wheat[J]. Chinese Journal of Eco-Agriculture, 2023, 31(12): 1997−2010 doi: 10.12357/cjea.20230229

Citation:

PDF( 4647 KB)

Meta analysis of the effects of foliar Se application on grain yield, protein content, and Se accumulation of winter wheat

doi: 10.12357/cjea.20230229

Institute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China

Funds: This study was supported by the National Key Research and Development Project of China (2021YFD1901005) and the Key Research and Development Project of Hebei Province (21326407D).

More Information

Corresponding author: JIA Liangliang, E-mail: jiall990@126.com; YUE Zengliang, E-mail: zlyue2203@163.com
Received Date: 2023-04-28
Accepted Date: 2023-09-04
Rev Recd Date: 2023-09-04

Available Online: 2023-10-07

Publish Date: 2023-12-15

Abstract

Abstract

To quantitatively analyze the impact of foliar Se application on grain yield, quality, and Se accumulation in winter wheat in China, 41 published studies (36 in Chinese and 5 in English) with a total of 379 pairs of samples were collected. A Meta-analysis was used to comprehensively analyze the effects of foliar Se application on the yield, grain protein, and Se content of winter wheat, and a subgroup analysis was used to evaluate the effects of different factors on response of winter wheat to foliar Se application, with no Se application as the control group and foliar Se application as the experimental group. The results showed that compared with no Se application, the yield, grain protein, and Se contents of winter wheat were increased by foliar Se application, with incremental rates of 3.80%, 2.44%, and 764.56%, respectively. In terms of the different regions, the effect of foliar Se application on yield and quality improvement was greater in the east and south than in the west and north, respectively. Overall, the effect of grain Se enrichment gradually decreased from west to east. In terms of foliar Se application management factors, it was most worthwhile to apply 15−60 g·hm⁻²once at the early filling stage or twice at the boot stage and early filling stage, which would meet the standard of Se enrichment and human needs. Soil fertility was an important factor influencing the effect of foliar Se application on the yield and quality of winter wheat. Soil contents of Se and total N had a significant effect on yield-increasing effect of foliar Se application. The effect of foliar Se application on yield improvement was the highest when the Se content of soil was between 0.2–0.4 mg·kg⁻¹. The effect of foliar Se application on winter wheat yield decreased with increasing total soil N. Soil Se, total N, available P, and available K contents significantly affected the effects of foliar Se application on protein content. The effect of foliar Se application on grain protein was higher when the soil Se content was between 0.2−0.4 mg·kg⁻¹, total N>1.5 g·kg⁻¹, available P>20 mg·kg⁻¹, and available K=100−200 mg·kg⁻¹. Soil fertility (soil Se content, organic matter content, and available K content) was the main factor affecting Se accumulation in grain when Se fertilizer was applied to the foliar. When the soil organic matter and available K contents were enhanced, foliar Se application had significantly increased the Se accumulation; however, the effect of Se accumulation caused by foliar Se application was high in Se-poor soil (<0.2 mg·kg⁻¹). Therefore, foliar Se application measures and soil conditions for optimization in different regions not only synergistically achieve the goals of high yields, good quality, and Se accumulation standards of wheat, but also reduce environmental contamination, which provides support for sustainable wheat production management with Se enrichment.
- Winter wheat,
- Selenium application,
- Yield,
- Protein content,
- Se accumulation,
- Meta-analysis

FullText(HTML)

References(62)

References

[1]	FAIRWEATHER-TAIT S J, BAO Y P, BROADLEY M R, et al. Selenium in human health and disease[J]. Antioxidants & Redox Signaling, 2011, 14(7): 1337−1383
[2]	CUBADDA F, AURELI F, CIARDULLO S, et al. Changes in selenium speciation associated with increasing tissue concentrations of selenium in wheat grain[J]. Journal of Agricultural and Food Chemistry, 2010, 58(4): 2295−2301 doi: 10.1021/jf903004a
[3]	WHO. Trace Element in Human Nutirtion and Health[M]. Geneva: World Health Organization, 1996: 105–120
[4]	李韬, 兰国防. 植物硒代谢机理及其以小麦为载体进行补硒的策略[J]. 麦类作物学报, 2012, 32(1): 173−177 LI T, LAN G F. Metabolism of selenium in plants and the strategies for selenium-biofortification using wheat[J]. Journal of Triticeae Crops, 2012, 32(1): 173−177
[5]	ZÖRB C, LUDEWIG U, HAWKESFORD M J. Perspective on wheat yield and quality with reduced nitrogen supply[J]. Trends in Plant Science, 2018, 23(11): 1029−1037 doi: 10.1016/j.tplants.2018.08.012
[6]	ROTRUCK J T, POPE A L, GANTHER H E, et al. Selenium: biochemical role as a component of glutathione peroxidase[J]. Nutrition Reviews, 1980, 38(8): 280−283
[7]	LEE S H, WOODARD H J, DOOLITTLE J J. Effect of phosphate and sulfate fertilizers on selenium uptake by wheat (Triticum aestivum)[J]. Soil Science and Plant Nutrition, 2011, 57(5): 696−704 doi: 10.1080/00380768.2011.623282
[8]	JÓŹWIAK W, POLITYCKA B. Effect of selenium on alleviating oxidative stress caused by a water deficit in cucumber roots[J]. Plants, 2019, 8(7): 217 doi: 10.3390/plants8070217
[9]	WHITE P J. Selenium accumulation by plants[J]. Annals of Botany, 2016, 117(2): 217−235
[10]	WANG Z J, GAO Y X. Biogeochemical cycling of selenium in Chinese environments[J]. Applied Geochemistry, 2001, 16(11/12): 1345−1351
[11]	刘慧, 杨月娥, 王朝辉, 等. 中国不同麦区小麦籽粒硒的含量及调控[J]. 中国农业科学, 2016, 49(9): 1715−1728 LIU H, YANG Y E, WANG Z H, et al. Selenium content of wheat grain and its regulation in different wheat production regions of China[J]. Scientia Agricultura Sinica, 2016, 49(9): 1715−1728
[12]	梁祎, 郝文琴, 石玉, 等. 不同光周期下叶面喷施纳米硒对生菜生长和品质的影响[J]. 中国生态农业学报(中英文), 2022, 30(1): 82−91 LIANG Y, HAO W Q, SHI Y, et al. Effects of nano-Se foliar spraying and photoperiod on lettuce growth and quality[J]. Chinese Journal of Eco-Agriculture, 2022, 30(1): 82−91
[13]	李文宗, 李春萍, 梁鑫, 等. 无人机叶面喷施梯度微肥对不同品种冬小麦籽粒矿质元素的影响[J]. 生物技术通报, 2021, 37(9): 152−160 LI W Z, LI C P, LIANG X, et al. Effects of foliar gradient micro-fertilizer sprayed by UAV on the grain mineral elements of different winter wheat varieties[J]. Biotechnology Bulletin, 2021, 37(9): 152−160
[14]	夏清. 不同粒色小麦籽粒产量及品质对外源硒的响应[D]. 太谷: 山西农业大学, 2019 XIA Q. Response of grain yield and quality to exogenous selenium of different colored-grain wheat[D]. Taigu: Shanxi Agricultural University, 2019
[15]	史芹, 高新楼. 不同时期喷施富硒液对小麦籽粒硒含量及产量的影响[J]. 山地农业生物学报, 2011, 30(6): 562−564 doi: 10.3969/j.issn.1008-0457.2011.06.019 SHI Q, GAO X L. Effect of application time of selenium fertilizer on wheat yield and grain selenium content[J]. Journal of Mountain Agriculture and Biology, 2011, 30(6): 562−564 doi: 10.3969/j.issn.1008-0457.2011.06.019
[16]	匡恩俊, 迟凤琴, 张久明, 等. 叶面喷硒对不同作物籽粒硒含量及产量的影响[J]. 中国土壤与肥料, 2018(4): 133−136 KUANG E J, CHI F Q, ZHANG J M, et al. The effect on crop selenium content and yield by foliar application of selenium[J]. Soil and Fertilizer Sciences in China, 2018(4): 133−136
[17]	冯魁, 贾莉莉, 贾永红, 等. 施硒对不同密度春小麦产量和籽粒硒含量的影响[J]. 麦类作物学报, 2018, 38(7): 834−840 FENG K, JIA L L, JIA Y H, et al. Effect of selenium application on yield and selenium content of spring wheat under different planting density[J]. Journal of Triticeae Crops, 2018, 38(7): 834−840
[18]	董石峰, 孙敏, 林文, 等. 拔节期喷施亚硒酸钠对小麦籽粒硒积累特性和产量的影响[J]. 山西农业科学, 2021, 49(4): 440−443 DONG S F, SUN M, LIN W, et al. Effects of spraying sodium selenite at jointing stage on grain selenium accumulation characteristics and yield of wheat[J]. Journal of Shanxi Agricultural Sciences, 2021, 49(4): 440−443
[19]	李文栓, 李晓军, 李培军, 等. 富硒营养液喷施量对春小麦产量和品质的影响[J]. 生态学杂志, 2020, 39(11): 3643−3650 LI W S, LI X J, LI P J, et al. Effects of foliar spray dose of selenium-rich nutrient solution on the yield and quality of spring wheat[J]. Chinese Journal of Ecology, 2020, 39(11): 3643−3650
[20]	蒋方山, 张海军, 吕连杰, 等. 叶面喷施亚硒酸钠对黑粒小麦籽粒硒含量、产量及品质的影响[J]. 麦类作物学报, 2018, 38(12): 1496−1503 JIANG F S, ZHANG H J, LYU L J, et al. Effect of foliar sodium selenite on selenium content, yield and quality in grains of black kernel wheat[J]. Journal of Triticeae Crops, 2018, 38(12): 1496−1503
[21]	刘鸣. 系统评价、Meta分析设计与实施方法[M]. 北京: 人民卫生出版社, 2011 LIU M. Systematic Evaluation, Meta-Analysis Design and Implementation[M]. Beijing: People’s Medical Publishing House, 2011
[22]	姜玲玲, 刘静, 赵同科, 等. 有机无机配施对番茄产量和品质影响的Meta分析[J]. 植物营养与肥料学报, 2019, 25(4): 601−610 doi: 10.11674/zwyf.18165 JIANG L L, LIU J, ZHAO T K, et al. Meta-analysis of combinative application of organic and inorganic fertilizers on effect of yield and qualities of tomato[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(4): 601−610 doi: 10.11674/zwyf.18165
[23]	孟轶, 廖萍, 魏海燕, 等. 施石膏对水稻产量和甲烷排放影响的荟萃分析[J]. 中国生态农业学报(中英文), 2023, 31(2): 280−289 doi: 10.12357/cjea.20220428 MENG Y, LIAO P, WEI H Y, et al. Effects of gypsum application on grain yield and methane emissions in rice paddies: a global meta-analysis[J]. Chinese Journal of Eco-Agriculture, 2023, 31(2): 280−289 doi: 10.12357/cjea.20220428
[24]	秦成, 裴红宾, 吴晓薇, 等. 外源硒对铅污染下荞麦生长及生理特性的影响[J]. 中国生态农业学报, 2015, 23(4): 447−453 QIN C, PEI H B, WU X W, et al. Effect of exogenous selenium on growth and development of buckwheat under plumbum stress[J]. Chinese Journal of Eco-Agriculture, 2015, 23(4): 447−453
[25]	郝敬爽. 叶面喷硒对不同小麦品种产量和籽粒品质的影响[D]. 杨凌: 西北农林科技大学, 2016 HAO J S. Effects of spraying selenium to different varieties of wheat on yield and quality[D]. Yangling: Northwest A & F University, 2016
[26]	张霞. 硒肥施用量及施用方式对小麦硒累积运转及土壤肥力的影响[D]. 太谷: 山西农业大学, 2019 ZHANG X. Effects of different selenium fertilizer amounts and application method on selenium accumulation transportation and soil fertility in wheat[D]. Taigu: Shanxi Agricultural University, 2019
[27]	周鑫斌, 施卫明, 杨林章. 富硒与非富硒水稻品种对硒的吸收分配的差异及机理[J]. 土壤, 2007, 39(5): 731−736 ZHOU X B, SHI W M, YANG L Z. Genotypical differences and characteristics of Se uptake and accumulation in rice[J]. Soils, 2007, 39(5): 731−736
[28]	芦文杰, 尹美强, 党芳芳, 等. 叶面喷硒对干旱胁迫下小麦幼苗生理特性的影响[J]. 山西农业科学, 2016, 44(12): 1780−1784 LU W J, YIN M Q, DANG F F, et al. Effect of selenium foliar spray on the physiological characteristics of wheat seedlings under drought stress[J]. Journal of Shanxi Agricultural Sciences, 2016, 44(12): 1780−1784
[29]	孙崇延, 李德安, 冯杰, 等. 施加硒化肥对麦粒的化学元素及氨基酸含量的影响[J]. 微量元素与健康研究, 1995, 12(3): 39−40 SUN C Y, LI D A, FENG J, et al. Effects of applying selenium fertilizer on chemical elements and amino acids in wheat grains[J]. Studies of Trace Elements and Health, 1995, 12(3): 39−40
[30]	刘庆, 田侠, 史衍玺. 施硒对小麦籽粒硒富集、转化及蛋白质与矿质元素含量的影响[J]. 作物学报, 2016, 42(5): 778−783 doi: 10.3724/SP.J.1006.2016.00778 LIU Q, TIAN X, SHI Y X. Effects of Se application on Se accumulation and transformation and content of gross protein and mineral elements in wheat grain[J]. Acta Agronomica Sinica, 2016, 42(5): 778−783 doi: 10.3724/SP.J.1006.2016.00778
[31]	赵淑章, 王绍中, 武素琴, 等. 小麦富硒研究概述与展望[J]. 中国农学通报, 2015, 31(24): 33−36 doi: 10.11924/j.issn.1000-6850.casb15010130 ZHAO S Z, WANG S Z, WU S Q, et al. Research progress and perspective on selenium-enriched wheat[J]. Chinese Agricultural Science Bulletin, 2015, 31(24): 33−36 doi: 10.11924/j.issn.1000-6850.casb15010130
[32]	成东梅, 尹国红, 赵伟峰, 等. 外源硒在绿色优质小麦生产中的应用研究[J]. 山西农业大学学报(自然科学版), 2021, 41(1): 20−29 CHENG D M, YIN G H, ZHAO W F, et al. Study on the application of exogenous selenium in the production of green and high-quality wheat[J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2021, 41(1): 20−29
[33]	白雪, 孙敏, 董石峰, 等. 富硒区补硒对黑小麦植株硒积累的影响[J]. 河北农业大学学报, 2020, 43(2): 34−39, 46 BAI X, SUN M, DONG S F, et al. Effects of selenium supplementation in Se-enriched areas on selenium accumulation of triticale plants[J]. Journal of Hebei Agricultural University, 2020, 43(2): 34−39, 46
[34]	黄婷苗, 于荣, 王朝辉, 等. 不同硒形态和施硒方式对小麦硒吸收利用的影响及残效[J]. 作物学报, 2022, 48(6): 1516−1525 doi: 10.3724/SP.J.1006.2022.11038 HUANG T M, YU R, WANG Z H, et al. Effects of different forms and application methods of selenium fertilizers on wheat selenium uptake and utilization and its residual availability[J]. Acta Agronomica Sinica, 2022, 48(6): 1516−1525 doi: 10.3724/SP.J.1006.2022.11038
[35]	YU T, YANG Z F, LYU Y Y, et al. The origin and geochemical cycle of soil selenium in a Se-rich area of China[J]. Journal of Geochemical Exploration, 2014, 139: 97−108 doi: 10.1016/j.gexplo.2013.09.006
[36]	王美珠, 章明奎. 我国部分高硒低硒土壤的成因初探[J]. 浙江农业大学学报, 1996, 22(1): 89−93 WANG M Z, ZHANG M K. A discussion on the cause of high-Se and low-Se soil formation[J]. Journal of Zhejiang Agricultural University, 1996, 22(1): 89−93
[37]	张忠, 周丽沂, 张勤. 硒的相态分析在环境地球化学监控网络研究中的应用[J]. 物探与化探, 1998, 22(3): 170−174 ZHANG Z, ZHOU L Y, ZHANG Q. The application of phase state analysis of selenium to the study of environmemtal geochemical moritoring network[J]. Geophysical and Geochemical Exploration, 1998, 22(3): 170−174
[38]	BARNEIX A J. Physiology and biochemistry of source-regulated protein accumulation in the wheat grain[J]. Journal of Plant Physiology, 2007, 164(5): 581−590 doi: 10.1016/j.jplph.2006.03.009
[39]	王琪. 水稻和小麦对有机硒的吸收、转运及形态转化机制[D]. 北京: 中国农业大学, 2017 WANG Q. Mechanisms of organic selenium uptake, translocation and speciation transformation in rice and wheat[D]. Beijing: China Agricultural University, 2017
[40]	黄青青. 水稻和小麦对硒的吸收、转运及形态转化机制[D]. 北京: 中国农业大学, 2015 HUANG Q Q. Mechanisms of selenium uptake, translocation and speciation transformation in rice and wheat[D]. Beijing: China Agricultural University, 2015
[41]	张妮, 李琦, 张栋, 等. 外源硒对滴灌小麦籽粒硒含量及产量的影响[J]. 麦类作物学报, 2015, 35(7): 995−1001 ZHANG N, LI Q, ZHANG D, et al. Effect of the selenium fertilizer in drip irrigation on wheat yield and selenium content[J]. Journal of Triticeae Crops, 2015, 35(7): 995−1001
[42]	邓洋. 硒营养素对旱地小麦籽粒硒含量及产量的影响[D]. 杨凌: 西北农林科技大学, 2018 DENG Y. Effects of selenium nutrition on selenium content and yield of dryland wheat grain[D]. Yangling: Northwest A & F University, 2018
[43]	宋家永, 王海红, 朱喜霞, 等. 叶面喷硒对小麦抗氧化性能及籽粒硒含量的影响[J]. 麦类作物学报, 2006, 26(6): 178−181 doi: 10.3969/j.issn.1009-1041.2006.06.040 SONG J Y, WANG H H, ZHU X X, et al. Effect of spraying selenium to the leaves on the performance of antioxidation of wheat and grain selenium content[J]. Journal of Triticeae Crops, 2006, 26(6): 178−181 doi: 10.3969/j.issn.1009-1041.2006.06.040
[44]	唐玉霞, 王慧敏, 吕英华, 等. 冬小麦硒素吸收积累特性及叶面喷硒效应的研究[J]. 华北农学报, 2010, 25(S1): 198−201 TANG Y X, WANG H M, LYU Y H, et al. Selenium absorption and accumulation characteristics of winter wheat and effects of foliar spray of selenium[J]. Acta Agriculturae Boreali-Sinica, 2010, 25(S1): 198−201
[45]	缪树寅. 不同品种小麦硒的耐受性差异及其动态吸收、转运规律研究[D]. 杨凌: 西北农林科技大学, 2013 MIAO S Y. Differences of phytotoxicity, tolerance, absorption and transport among several varieties of wheat when amended selenate and selenite[D]. Yangling: Northwest A & F University, 2013
[46]	于丽敏, 薛艳芳, 高华鑫, 等. 小麦富硒研究进展[J]. 山东农业科学, 2015, 47(6): 137−144 doi: 10.14083/j.issn.1001-4942.2015.06.033 YU L M, XUE Y F, GAO H X, et al. Research progress of Se-enriched wheat[J]. Shandong Agricultural Sciences, 2015, 47(6): 137−144 doi: 10.14083/j.issn.1001-4942.2015.06.033
[47]	陈泊宁. 冬小麦施硒技术及施硒效果探究[D]. 武汉: 华中农业大学, 2019 CHEN B N. Research on the effect of selenium application technology in winter wheat[D]. Wuhan: Huazhong Agricultural University, 2019
[48]	STATWICK J, SHER A A. Selenium in soils of western Colorado[J]. Journal of Arid Environments, 2017, 137: 1−6 doi: 10.1016/j.jaridenv.2016.10.006
[49]	LYONS G, STANGOULIS J, GRAHAM R. High-selenium wheat: biofortification for better health[J]. Nutrition Research Reviews, 2003, 16(1): 45−60 doi: 10.1079/NRR200255
[50]	周鑫斌, 于淑慧, 谢德体. pH和三种阴离子对紫色土亚硒酸盐吸附-解吸的影响[J]. 土壤学报, 2015, 52(5): 1069−1077 doi: 10.11766/trxb201409290496 ZHOU X B, YU S H, XIE D T. Effects of pH and three kinds of anions on selenium absorption and desorption in purple soil[J]. Acta Pedologica Sinica, 2015, 52(5): 1069−1077 doi: 10.11766/trxb201409290496
[51]	JOHNSON C C, GE X, GREEN K A, et al. Selenium distribution in the local environment of selected villages of the Keshan Disease belt, Zhangjiakou District, Hebei Province, People’s Republic of China[J]. Applied Geochemistry, 2000, 15(3): 385−401 doi: 10.1016/S0883-2927(99)00051-7
[52]	李永华, 王五一, 杨林生, 等. 陕南土壤中水溶态硒、氟的含量及其在生态环境的表征[J]. 环境化学, 2005, 24(3): 279−283 LI Y H, WANG W Y, YANG L S, et al. Concentration and environmental significance of water soluble-Se and water soluble-F in soils of South Shaanxi Province[J]. Environmental Chemistry, 2005, 24(3): 279−283
[53]	GOH K H, LIM T T. Geochemistry of inorganic arsenic and selenium in a tropical soil: effect of reaction time, pH, and competitive anions on arsenic and selenium adsorption[J]. Chemosphere, 2004, 55(6): 849−859 doi: 10.1016/j.chemosphere.2003.11.041
[54]	迟凤琴, 徐强, 匡恩俊, 等. 黑龙江省土壤硒分布及其影响因素研究[J]. 土壤学报, 2016, 53(5): 1262−1274 doi: 10.11766/trxb201511300524 CHI F Q, XU Q, KUANG E J, et al. Distribution of selenium and its influencing factors in soils of Heilongjiang Province, China[J]. Acta Pedologica Sinica, 2016, 53(5): 1262−1274 doi: 10.11766/trxb201511300524
[55]	TOLU J, THIRY Y, BUENO M, et al. Distribution and speciation of ambient selenium in contrasted soils, from mineral to organic rich[J]. Science of the Total Environment, 2014, 479/480: 93−101 doi: 10.1016/j.scitotenv.2014.01.079
[56]	戴慧敏, 宫传东, 董北, 等. 东北平原土壤硒分布特征及影响因素[J]. 土壤学报, 2015, 52(6): 1356−1364 DAI H M, GONG C D, DONG B, et al. Distribution of soil selenium in the Northeast China Plain and its influencing factors[J]. Acta Pedologica Sinica, 2015, 52(6): 1356−1364
[57]	FU M M, HUANG B, JIA M M, et al. Effect of intensive greenhouse vegetable cultivation on selenium availability in soil[J]. Pedosphere, 2015, 25(3): 343−350 doi: 10.1016/S1002-0160(15)30002-3
[58]	JOHNSSON L. Selenium uptake by plants as a function of soil type, organic matter content and pH[J]. Plant and Soil, 1991, 133(1): 57−64 doi: 10.1007/BF00011899
[59]	刘大会, 周文兵, 朱端卫, 等. 硒在植物中生理功能的研究进展[J]. 山地农业生物学报, 2005, 24(3): 253−259 doi: 10.3969/j.issn.1008-0457.2005.03.015 LIU D H, ZHOU W B, ZHU D W, et al. General survey on physiological function of selenium in plant[J]. Journal of Mountain Agriculture and Biology, 2005, 24(3): 253−259 doi: 10.3969/j.issn.1008-0457.2005.03.015
[60]	WANG Q Y, ZHANG J B, ZHAO B Z, et al. Influence of long-term fertilization on selenium accumulation in soil and uptake by crops[J]. Pedosphere, 2016, 26(1): 120−129 doi: 10.1016/S1002-0160(15)60028-5
[61]	HAMDY A A, NIELSEN G G. Fractionation of soil selenium[J]. Journal of Plant Nutrition and Soil Science, 1976, 6: 697−703
[62]	邢丹英, 许少华, 高剑华, 等. 不同硒源后效应对小麦农艺性状与富硒量的影响[J]. 湖北农业科学, 2010, 49(12): 3009−3010, 3014 XING D Y, XU S H, GAO J H, et al. After-effects of different selenium sources on the agronomic characteristics and selenium contents of wheat[J]. Hubei Agricultural Sciences, 2010, 49(12): 3009−3010, 3014