Effect of insect-resistant transgenic rice and its hybrid combination rice on diversity and composition of soil microbial community

SONG Yana; CHEN Zaijie; LIN Yan; HU Taijiao; WU Mingji; WANG Feng

doi:10.12357/cjea.20230267

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SONG Y N, CHEN Z J, LIN Y, HU T J, WU M J, WANG F. Effect of insect-resistant transgenic rice and its hybrid combination rice on diversity and composition of soil microbial community[J]. Chinese Journal of Eco-Agriculture, 2024, 32(1): 15−29 doi: 10.12357/cjea.20230267

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Effect of insect-resistant transgenic rice and its hybrid combination rice on diversity and composition of soil microbial community

doi: 10.12357/cjea.20230267

Institute of Biological Technology, Fujian Academy of Agricultural Sciences / Fujian Provincial Key Laboratory of Genetic Engineering for Agriculture, Fuzhou 350003, China

Funds: This study was supported by Fujian Provincial Public Welfare Research Institute Special Project (2021R1027002), The ‘5511’ Collaborative Innovation Project for High-quality Development and Surpasses of Agriculture between Government of Fujian Province and Chinese Academy of Agricultural Sciences (XTCXGC2021002), and Fujian Province Science and Technology Major Project (2020NZ08017).

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Corresponding author: E-mail: wf@fjage.org
Received Date: 2023-05-15
Accepted Date: 2023-08-21

Available Online: 2023-10-16

Abstract

Abstract

Microorganisms drive the soil material cycle and evolution of fertility, and their community composition is related to the stability and sustainability of the soil microecosystem. The study of changes in the soil microbial community in insect-resistant transgenic rice is an important part of environmental safety assessments. Based on high-throughput sequencing of the bacterial 16S rRNA and fungal ITS genes, this study analyzed the differences in diversity and composition of microbial communities in paddy soils with insect-resistant transgenic rice ‘MFB’, insect-resistant transgenic hybrid rice ‘Minfeng A/MFB’ ‘Tianfeng A/MFB’ or ‘Gufeng A/MFB’ and non-transgenic conventional rice ‘Minhui 3301’ or hybrid rice ‘Tianyouhuazhan’. The results showed that, compared with non-transgenic conventional rice ‘Minhui 3301’ or hybrid rice ‘Tianyouhuazhan’, both insect-resistant transgenic rice ‘MFB’ and transgenic hybrid rice ‘Minfeng A/MFB’ ‘Tianfeng A/MFB’ or ‘Gufeng A/MFB’ could significantly increase the yield (P<0.05). At the same time, high-throughput sequencing showed that the α-diversity indexes of Chao1, Observed_species, and Shannon of bacterial or fungal communities in paddy soil with ‘MFB’ were higher than those in soil with ‘Minhui 3301’ at all stage except for fungal communities at rice maturation stage, and the differences at rice maturation or tillering stage are significant (P<0.05). At the heading stage of rice, the values of α-diversity indexes of Shannon of bacterial or fungal communities in paddy soils with the insect-resistant transgenic hybrid rice ‘Minfeng A/MFB’ ‘Tianfeng A/MFB’ or ‘Gufeng A/MFB’ were between those of soils with ‘MFB’ and ‘Tianyouhuazhan’. The results of β-diversity analysis of bacterial or fungal communities showed that there were no significant differences in the composition of microbial communities in paddy soils with different varieties of rice in this field experiment. However, the relative abundance of Proteobacteria, which showed the highest abundance of bacteria, in paddy soil with ‘MFB’ increased compared with that of ‘Minhui 3301’, and reached the significance level at the tillering and maturation stage of rice (P<0.05). In contrast, the relative abundance of Ascomycota, which showed the highest abundance of fungi, was reduced in paddy soil with ‘MFB’ and reached the significance level at the tillering and heading stages of rice (P<0.05). At the heading stage of rice, the relative abundances of Proteobacteria and Ascomycota in paddy soils with ‘Minfeng A/MFB’ ‘Tianfeng A/MFB’ or ‘Gufeng A/MFB’ were between those of soils with ‘MFB’ and ‘Tianyouhuazhan’. According to the functional prediction of microbial communities, the differences in functional composition of bacterial communities in paddy soil between soils with ‘MFB’ and ‘Minhui 3301’ gradually increased with rice growth. In summary, insect-resistant transgenic rice and its transgenic hybrid rice increased the diversity of soil bacterial and fungal communities and changed the relative abundance of major bacterial and fungal species with increased yield, but did not have significant effects on the community and functional composition of bacteria or fungi.
- Insect-resistant transgenic rice,
- Bacterial community,
- Fungal community,
- High throughput sequencing

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