Plant genetic diversity of ecotourism rural landscape

FENG Zhi; YAO Zhi; SUN Quan; LI Meng; WU Xiuzhong; CHEN Dahua; JIANG Long; FAN Kaifang; TANG Jianlu; LI Kai; MI Xiaoqin; YU Yong; WANG Yiqiang

doi:10.12357/cjea.20230326

Volume 31 Issue 12

Dec. 2023

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Article Navigation > Chinese Journal of Eco-Agriculture > 2023 > 31(12): 1896-1908

FENG Z, YAO Z, SUN Q, LI M, WU X Z, CHEN D H, JIANG L, FAN K F, TANG J L, LI K, MI X Q, YU Y, WANG Y Q. Plant genetic diversity of ecotourism rural landscape[J]. Chinese Journal of Eco-Agriculture, 2023, 31(12): 1896−1908 doi: 10.12357/cjea.20230326

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Plant genetic diversity of ecotourism rural landscape

doi: 10.12357/cjea.20230326

FENG Zhi^{1, 2
,},
YAO Zhi^{1, 2},
SUN Quan^{1, 2},
LI Meng^{1, 2},
WU Xiuzhong³,
CHEN Dahua³,
JIANG Long³,
FAN Kaifang^{1, 2},
TANG Jianlu^{1, 2},
LI Kai^{1, 2},
MI Xiaoqin⁴,
YU Yong⁴,
WANG Yiqiang^{1, 2
,
,}

1.
Hunan Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha 410004, China
2.
Yuelushan Laboratory of Carbon Sinks Forests Variety Innovation Center, Changsha 410004, China
3.
Forestry Bureau of Xiangxi Autonomous Prefecture of Hunan Province, Jishou 416000, China
4.
Xiangxi Autonomous Prefecture Forest Ecological Experimental Station of Hunan Province, Yongshun 416700, China

Funds: The study was supported by the National Natural Science Foundation of China (32171842), the National Key Research and Development Program of China (2019YFD11004031), and the Innovation Project of Central South University of Forestry and Technology (90102-63223068).

More Information

Corresponding author: E-mail: wangyiqiang12@163.com
Received Date: 2023-06-12
Accepted Date: 2023-09-01

Available Online: 2023-10-26

Publish Date: 2023-12-15

Abstract

Abstract

The incorporation of rural landscape plants plays a pivotal role in the development and establishment of ecotourism. By studying the genetic diversity of plants in rural landscapes, the richness of genetic variation and the stability of the genetic structure within a population can be revealed. This serves as an important theoretical foundation for the construction of rural ecological landscapes and biodiversity maintenance and is of great significance for the construction of beautiful villages. In this study, eight native landscape species were selected from two ecotourism villages (Changkou Village, Sanming City, Fujian Province; and Paifang Community, Nanjing City, Jiangsu Province). Native rural landscape plant samples were collected from the whole village area. Phenotypic characteristics were measured, and ISSR-PCR experiments were performed. Through analysis of phenotypic features and detection of molecular markers, the Shannon index and Nei’s genetic diversity indexes were calculated to elucidate the levels of genetic diversity of native landscape plant species in different areas. Cluster analysis using phenotypic features identified five types of Liquidambar formosana, six types of Cyclobalanopsis chungii, four types of Quercus glauca, sixteen types of Zelkova serrata, seven types of Toona sinensis, ten types of Aster indicus, five types of Chrysanthemum indicum, and six types of Rubus hirsutus. The phenotypic coefficient variation and Shannon index of the eight native landscape plant species ranged from 0.23 to 0.58, and from 1.51 to 6.74, respectively. In the total area, artificial area and natural area, the Nei’s genetic diversity indexes of the eight native landscape plant species ranged from 0.240 to 0.536, 0.244 to 0.540, and 0.193 to 0.367, respectively. For the eight native landscape plant species, the percentage of polymorphic loci varied from 45.00% to 100.00%, the number of alleles varied from 1.45 to 2.00, and the number of effective alleles varied from 1.30 to 1.64. The results revealed that the phenotypic (Shannon index) and molecular (Nei’s genetic diversity index) genetic diversity levels of the eight native landscape plant species were higher than the average diversity level in numerous other landscape plant species. Additionally, the rural landscape plant species exhibited abundant genetic variation. The genetic diversity of certain rural landscape plant species exhibited a notable degree of variability; however, there were significant differences in the levels of genetic diversity observed between natural and artificial areas. In the context of rural landscape construction, it is important to prioritize the assessment of genetic diversity in rural landscape plant populations. Appropriate measures should be implemented to enhance the even distribution of genetic polymorphisms within the population and preserve the genetic diversity of native landscape plant species. This approach is essential to ensure the long-term stability of rural ecological landscapes.
- Ecotourism village,
- Rural landscape plants,
- Genetic diversity,
- Phenotypic Shannon index,
- Nei’s genetic diversity index

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References(55)

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